[Histonet] CD98 and PLGF

[Featherstone, Annette]

YES!!I am! Or was...
I used a pepsin pretreatment @37* for 15 minutes, Block with a protein
block, primary antibody 1:100 for 1hr, Goat secondary 30 min rt, Alk phos 30
minutes rt, and whatever chromgen you use for alk phos, rt 40 min. My Plgf
is from Santa Cruz.
Annette Featherstone HT/MLT

-----Original Message-----
From: Patsy Ruegg [mailto:pruegg <@t> colobio.com]
Sent: Friday, March 19, 2004 13:58
To: Patsy Ruegg; Histonet <@t> Pathology. Swmed. Edu
Cc: Ihcrg <@t> Yahoogroups. Com
Subject: RE: [Histonet] CD98 and PLGF


I guess no one is using cd98 and/or Placental Growth Factor on ffpe
tissue????

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Patsy
Ruegg
Sent: Wednesday, March 17, 2004 10:03 AM
To: Histonet <@t> Pathology. Swmed. Edu
Cc: Ihcrg <@t> Yahoogroups. Com
Subject: [Histonet] CD98 and PLGF


I am seeking those who may have experience with cd98 (h-300) Santa Cruz-9160
and/or Abcam anti-PLGF ab9542 IHC on ffpe tissue???  Do these antibodies
work in ffpe tissue?
Patsy


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