[Histonet] Phosphotungstic Acid Hematoxylin stain without usingZenker's

[Lee & Peggy Wenk]

We've postmordanted in Bouins in 60 degree C. oven for 1 hour. Wash in
running water until yellow is gone. Then stain as usual. The striated muscle
fibers seemed less "splotchy" to me with this method than with
postmordanting in Zenker or saturated mecuric chloride.

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073

----- Original Message -----
From: "Boswell" <kcboswell <@t> grandecom.net>
To: <Histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, March 19, 2004 3:29 PM
Subject: [Histonet] Phosphotungstic Acid Hematoxylin stain without
usingZenker's


Does anyone have a procedure for Phosphotungstic Acid Hematoxylin stain
without using Zenker's.  We have looked everywhere and are unable to find
it.  Thank you.
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