[Histonet] Using H & E slides on the Benchmark XT

Joe Nocito JNocito <@t> Pathreflab.com
Fri Mar 19 06:51:05 CST 2004


Morning Histoland,
	We have acquired the Ventana Benchmark XT and I really need some help. When
we were performing the slides manually, we were able to take an H&E slide,
take off the coverslip and run an immuno on it.  The doctors fell in love
with this since we processes a lot of cervical biopsies and sometimes the
lesion is not there on recuts. We have had a problem with H&E slides not
picking up the immuno staining. I set up different programs using the wet
slide protocol, but I'm having problems still.
	Would anyone be willing to share their protocol with me?

Thanks

Joe Nocito, BS, HT (ASCP) QIHC
Histology Manager
Pathology Reference Lab
San Antonio, TX 78205


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