[Histonet] iron stain on bone marrow-SOLUTIONS

[Dunikoski, Leonard PhD]

"Remember the order: acid to water"

-----Original Message-----
From: Bonner, Janet [mailto:Janet.Bonner <@t> FLHOSP.ORG] 
Sent: Thursday, March 18, 2004 12:56 PM
To: 'Wendy England'; histonet <@t> pathology.swmed.edu
Subject: RE: [Histonet] iron stain on bone marrow-SOLUTIONS


The 20% Hydrochloric acid solution should be added to the 10% Potassium
ferrocyanide solution - (not 10% potassium ferrocyanide to 20% Hydrochloric
acid solution) when making the 1:1 mixture.


-----Original Message-----
From: Wendy England [mailto:weneng <@t> hotmail.com]
Sent: Wednesday, March 17, 2004 3:28 PM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] iron stain on bone marrow


Thanks again to everybody who gave me the information about this stain! Here
comes another question: I couldn't see much iron on my slide, only 
about 2~3 spots per rat bone marrow slide. I fixed them in Methanol for 15 
min, then in freshly mixed stain solution(equal part of 20% HCl and 10% 
potassium ferrocyanide) for 30 min in 50-60C. Then counterstain in Safranin 
after wash. Then wash, air dry then mount.

I was told there should be much more iron. I feel bad now. Could any body 
give me advice or could I ask if it's possible due to the bone marrow 
preparation  since we indeed saw some spots. I didn't prepare the sample.

Thanks and look forward to hear from you there!

Wendy

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