[Histonet] Please Help with Cresyl Violet Acetate

[Featherstone, Annette]

Our Cresyl Echt Violet is
          CEV Stock Sol.  CI-51010 --5gm
                          D. H2O----80 ml
                          Absolute--20 ml
             Warm the distilled, add the CEV mix, then add alcohol.
Working CEV solution
              45 ml of stock
             Acetic Acid, glacial--15 drops
Deparaffinize, stain in working sol for 8 minutes, dehydrate, clear.

Nissl substance and nuclei----Blue-purple
Background--------------------Colorless

Annette Featherstone HT/MLT

-----Original Message-----
From: Sebree Linda A. [mailto:la.sebree <@t> hosp.wisc.edu]
Sent: Thursday, March 18, 2004 11:43
To: Histonet (E-mail)
Subject: [Histonet] Please Help with Cresyl Violet Acetate 


Good Morning Histoneters,
I am trying to work-up a CEVstain for nissel  substance and I am having
difficulties producing a magenta effect on the nissel. I have tried several
powders with the CI # of 10510-54-0 along with different staining
procedures.  I would appreciate any help or suggestions anyone has to offer.


Angela Baker

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Clinical & Research Laboratory
DM223-VA
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX:  (608)262-7174



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