[Histonet] xylene substitute

[Gladney, Diane C Ms MACH]

Eddy,

I have used Thermo Electron (formerly Shandon) Xylene Substitute in my
auto-stainer for years without any problems. BUT I only use it in the
deparaffinization at the beginning of the staining series. I use Xylene in
the last three stations of the stainer. Our mounting medium has xylene as
the solvent so the substitute is not compatible with the mounting medium. I
have tried using the xylene substitute mounting media and I do not like the
results. Therefore, we stay with a good old tried and true mounting medium.
At least we have considerably cut our use of Xylene as we also use the same
xylene substitute in our processors. If you have any other questions, please
feel free to contact me.

Happy Cutting,
Diane

Diane C. Gladney, HT (ASCP)
Histology /Cytology Supervisor
Moncrief Army Community Hospital
P.O. BOX 484
4500 Stuart Ave.
FT. Jackson, SC 29207

(803) 751-2530
DSN 734-2530

EMAIL: diane.gladney <@t> se.amedd.army.mil  OR
                 dcgx1 <@t> aol.com


-----Original Message-----
From: Eddie Marquez [mailto:EMarquez <@t> crch.hawaii.edu]
Sent: Monday, March 15, 2004 3:19 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] xylene substitute



Aloha from Hawaii,

Just needed some info.-Has anybody used xylene substitute with a linear
stainer; for standard H&E's?  If so, how good are the results? May I also
have the brand name.

Thank you,

Eddie from the Cancer Research 


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