Fwd: [Histonet] re: cryostat sectioning

[Atoska S. Gentry]

Please pardon my ignorance, but what kind of frozen tissues are you 
sectioning on the sliding microtome, what brand & type sliding microtome 
are you using, and how are you keeping your samples frozen for sectioning 
on it? Thanks, Atoska


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>From: "Mollie Lange" <lange <@t> kennedykrieger.org>
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>Hi Kathleen,
>
>I feel your pain.  I've been cutting cryostat sections for almost 30
>years, and sometimes it can be completely maddening for no discernible
>reason.
>
>It could possibly be static as one Histonetter suggested.  A Japanese
>fellow in our lab showed us that spraying Cytocool on the knife and roll
>plate really works.  It's the ONLY thing that consistently works.
>
>Are your tissue blocks cryoprotected in sucrose?  Have you tried a
>freshly made solution of your cryoprotectant in case the original was
>not made proplerly?
>
>We are a research lab, and we always cut our fixed frozen sections for
>immuno on a sliding microtome.  We find this method much easier than the
>cryostat for fixed frozen. Do you have a sliding microtome available and
>have you tried it?
>
>Good luck,
>Mollie Lange
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Atoska S. Gentry B.S., HT(ASCP)
Research Assistant III
Scott-Ritchey Research Center
College of Veterinary Medicine
Auburn University, AL  36849
Phone# (334)844-5579  Fax# (334)844-5850