[Histonet] TUNEL (PROMEGA)

[hanim shueb]

I'm  doing TUNEL staining  on mouse brains (formaldehyde fixed, paraffin 
section) right now.

I have problem with non-specific binding of the cells; ie almost all cells 
stained positive. I used the protocol given by Promega with some 
modifications; I performed microwave antigen retrieval and block the tissues 
with 20% NGS/1%BSA in PBS before adding TdT reaction mix.

Can anyone help me to solve the non-specific bindings I keep getting?

many thanks,
Hanim Shueb
Western Australia

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