[Histonet] Monkey lung question

[Jennifer Sipes]

I was wondering if anyone could help me with my last question of the day:
 
 
A girl in my lab is cryo fixing monkey lung.  The procedure goes as 
follows:
          Each lung is sectioned into 9 parts.  Of the total 18 lung 
sections, only 5% of them go for cryosectioning.  They are interested in the the 
large airways.  She is having a problem with getting decent cuts.  Currently, she 
is submerging the small pieces into OCT overnight at 4 C  then mounting them.  Does 
anyone happen to have a protocol better than that.  I told her that that was the 
incorrest way, but she said that was the protocol she was given.  Please remember that 
the rest of the tissue is going for Real Time PCR, RNA extraction, etc. 
 
I would greatly appreciate any information that you folks could give 
me.  Thank you for your time.


		
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