[Histonet] 2 questions

[Jennifer Sipes]

My name is Jennifer.  I have recently taken on the task of doing histology for my lab.  I have just 2 questions:
 
1.  Does anyone have a protocol for Toludine Blue staining?
 
2.  A girl in my lab is cryo fixing monkey lung.  The procedure goes as follows:
          Each lung is sectioned into 9 parts.  Of the total 18 lung sections, only 5% of
          them go for cryosectioning.  They are interested in the the large airways.
     She is having a problem with getting decent cuts.  Currently, she is submerging the
     small pieces into OCT overnight at 4 C  then mounting them.  Does anyone happen
     to have a protocol better than that.  I told her that that was the incorrest way, but she
     said that was the protocol she was given.  Please remember that the rest of the
     tissue is going for Real Time PCR, RNA extraction, etc. 
 
I would greatly appreciate any information that you folks could give me.  Thnak you for your time.
 
 


Jennifer K. Sipes
Sr. Laboratory Technician
Johns Hopkins University
Ross Research Building Rm 929
720 Rutland Avenue
Baltimore, MD  21205
phone:  410-614-0131
cell:     443-413-0853
fax:     410-955-9677
e-mail:  jengirl1014 <@t> yahoo.com
 






		
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