[Histonet] IHC after haematoxylin[Scanned]

[Sebree Linda A.]

Dave,

We've infrequently had to IHC stain over an H&E.  Don't bother to destain, it all usually comes out during pretreatment (HIER or enzyme).

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Clinical & Research Laboratory
DM223-VA
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX:  (608)262-7174



-----Original Message-----
From: David Rushworth [mailto:David.Rushworth <@t> mail.bhrv.nwest.nhs.uk]
Sent: Wednesday, June 30, 2004 4:17 AM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] IHC after haematoxylin[Scanned]


One of my colleagues inadvertently stained the batch of IHC slides with HX
for about 3 mins whilst taking them to water prior to IHC. My question is
will this affect subsequent IHC? Do we decolourise etc. Any feedback much
appreciated. Dave.

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