[Histonet] IHC after haematoxylin[Scanned]

[Tom McNemar]

Should be no problem at all.  We often do IHC (DAB) over h&e.  No
decolorization needed.

Tom Mc Nemar HT(ASCP)
Histology Supervisor
Licking Memorial Hospital
Newark, Ohio 43055



-----Original Message-----
From: David Rushworth [mailto:David.Rushworth <@t> mail.bhrv.nwest.nhs.uk] 
Sent: Wednesday, June 30, 2004 5:17 AM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] IHC after haematoxylin[Scanned]


One of my colleagues inadvertently stained the batch of IHC slides with HX
for about 3 mins whilst taking them to water prior to IHC. My question is
will this affect subsequent IHC? Do we decolourise etc. Any feedback much
appreciated. Dave.

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