[Histonet] cryosections problem

[Gareth Davis]

I usually use a 6 well culture dish (for rat brains) or a smaller dish for mouse.  I either set the dish on ice, sometimes with a paper towel on top of the ice.  Or, in one lab we had cold plates, which can be ordered from VWR.  They are basically plates with the freezing material inside, and they kept in the freezer until ready to use.  Let me know if this works for you.
Gareth Davis


Inga Hansson <Inga.Hansson <@t> neuro.uu.se> wrote:
Ok.....that was new! How cold do you keep the PBS? Just above freezing?

Thanks a lot!

Inga
-- 


Inga Hansson
dept. neuroscience, div. neurobiology
PO Box 587
Biomedical Centre
Husargatan 3
S-751 23
Uppsala
SWEDEN

phone:+46-18-4714384
fax: +46-18-559017

		
---------------------------------
Do you Yahoo!?
Take Yahoo! Mail with you! Get it on your mobile phone.