[Histonet] cryosections problem

[Inga Hansson]

Hi everyone!

I´m trying to section PFA-fixed and sucrose protected mouse brain. 
The sections curl and break when I try to put them on the glass. I 
don´t know what temperature to use; is -23 too low? Should I set the 
same temperature on the knife as the specimen? Cold or warm slides?

Any suggestions are gratefully appreciated!!

Thanks!

Inga
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Inga Hansson
dept. neuroscience, div. neurobiology
PO Box 587
Biomedical Centre
Husargatan 3
S-751 23
Uppsala
SWEDEN

phone:+46-18-4714384
fax: +46-18-559017