[Histonet] Pollak's trichrome

[Smith, Allen]

Gabe's trichrome is easier than Pollak's trichrome, does not require mercury
fixation, and almost always gives excellent results. It is given in
Kiernan's HISTOLOGIC AND HISTOCHEMICAL METHODS.

Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University
School of Graduate Medical Sciences
            Podiatric Medicine and Surgery
Miami Shores, Florida


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Geoff
McAuliffe
Sent: Thursday, June 24, 2004 6:04 PM
To: Bruce W Brodersen
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Pollak's trichrome



A friend of mine tried this many years ago and never could get good 
colors. He went back to longer methods, but they worked.

Geoff

Bruce W Brodersen wrote:

>We've tried using the procedure listed on:
>http://members.pgonline.com/~bryand/StainsFile/stain/conektv/tri_pollak.htm
>
>We keep having collagen stain green rather than blue, as the procedure
>describes.  Any suggestions as to what we are doing wrong?
>Thanks.
>
>Bruce W. Brodersen, DVM, PhD
>University of Nebraska Veterinary Diagnostic Center
>Fair St. & E. Campus Loop
>Lincoln, NE  68583-0907
>
>voice (402) 472-1434
>FAX (402 472-3094
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>  
>

-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff <@t> umdnj.edu
**********************************************




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