[Histonet] Histosearch/Help with bone HIER

[Patsy Ruegg]

Yes I used 5% elmers glue coated slides for really difficult bone section
adherence but for IHC you have to be careful depending on which marker you
are using there may be non-specific binding to the glue (it is especially
the case with collagen markers as you can imagine).
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of DiCarlo,
Margaret
Sent: Friday, June 25, 2004 7:06 AM
To: 'Barbara Lentz'; Jackie.O'Connor <@t> abbott.com;
histonet <@t> lists.utsouthwestern.edu;
histonet-bounces <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Histosearch/Help with bone HIER


Jackie,

You can make up a 5% solution from titebond II (glue) purchased from any
hardware store.  I use it for my bone sections but I know the pathology
supervisor borrowed some of my solution for H & E's and immuno's and has had
excellent results with the tissue staying on.  It is worth a try.  Feel free
to contact me if you have any questions.  Good luck.

Peggy DiCarlo HT (ASCP)
Orthopedics Bone Lab
Buffalo General Hospital
100 High St.
Buffalo, NY  14203
716-859-1293



-----Original Message-----
From: Barbara Lentz [mailto:Barbara_Lentz <@t> dahlchase.com]
Sent: Friday, June 25, 2004 08:28
To: Jackie.O'Connor <@t> abbott.com; histonet <@t> lists.utsouthwestern.edu;
histonet-bounces <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Histosearch/Help with bone HIER


I place the slides in NBF for 30 minutes prior to HIER.  This seems to
help keep the tissue from falling off.  Hope this helps your procedure,
to.  Barb

>>> <Jackie.O'Connor <@t> abbott.com> 06/24/04 01:54PM >>>
Is anyone else having trouble searching the archives today?  It keeps
freezing up on me.  (It's only 51F in Chicago today - hmmmmm)

I was looking for some tips on how to keep #?+$% FFPE bone from falling

off the slide during HIER.

Tibiae are decalcifed in 5% formic acid, cut at 5 microns, dried flat
at
37C overnight.  The marrow stays on but I'm losing the bone.

I know someone has a sure-fire answer.

Jackie O'
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