[Histonet] PLP fixative

[Veronique Andriessen]

Hi Histonetters,

It has been some time, but I would like to thank everyone for their kind
replies on my last post on doublestaining for somatostatin receptor and
desmin.
I have tried the PLP fixative and it gives the best staining for the
somatostatin receptor until now. Desmin works pretty well with a bit of
retrieval.
I've seen some inconsistancy in retrieval time so I'm wondering if my tissue
is completely fixed. Does anybody know how long tissue must stay in PLP
fixative (after perfusion) to be completely fixed?

Thanks

Veronique Andriessen BAS
Lab. Molecular Liver Cell Biology,
Free University Brussels (VUB)
Belgium