[Histonet] RE: Histonet Digest, Vol 7, Issue 16
Dorothy.L.Webb <@t> HealthPartners.Com
Dorothy.L.Webb <@t> HealthPartners.Com
Wed Jun 23 10:40:43 CDT 2004
In regard to the staffing recommendations for histology labs, CAP has
established guidelines that were put out in the NSH edition in 2002 and are
as follows: Each trained histotech can be expected can be expected tp
produce about 12,000 slides per year. This would break down to 10,000 H&E's
and 2,000 special stains per year. I would be happy to fax this report to
anyone who would like to see the total guidelines suggested and the
breakdown. My E-Mail address is dorothy.l.webb <@t> healthpartners.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
histonet-request <@t> lists.utsouthwestern.edu
Sent: Saturday, June 12, 2004 12:03 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 7, Issue 16
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Today's Topics:
1. Per Diem Histotech Position in Southern California
(Laurie Colbert)
2. Frozen tissue to be embedded in OCT later (Gayle Callis)
3. JCAHO (Demarinis, Carolyn)
4. Re: (no subject) (mari.ann.mailhiot <@t> leica-microsystems.com)
5. Histology exam blocks (Poteete, Jacquie A.)
6. FW: [Histonet] cd138 vendor (Patti Loykasek)
7. Rabbit Eyes (Boone, Linda (HSC))
8. unsubscribe (DiCarlo, Margaret)
9. RE: JCAHO (Bartlett, Jeanine)
10. cassette labeler (Stacey Burton)
11. best microtomes (Tom Wells)
----------------------------------------------------------------------
Message: 1
Date: Fri, 11 Jun 2004 10:17:52 -0700
From: "Laurie Colbert" <laurie.colbert <@t> huntingtonhospital.com>
Subject: [Histonet] Per Diem Histotech Position in Southern California
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<0BE6ADFAE4E7E04496BF21ABD34662801D0CD6 <@t> EXCHANGE1.huntingtonhospital.com>
Content-Type: text/plain; charset="iso-8859-1"
Huntington Memorial Hospital in Pasadena, CA is looking for a per diem
histotech to fill in for vacation and sick leave. The hours could vary
from 3:00 am to 5:00 am start time, Tuesday-Saturday.
I am also looking for someone to fill in for a tech that is going out on
Maternity Leave from the end of September to the end of January. This would
be a Tuesday-Saturday position starting at 3:30 am during the week and 2:30
am on Saturdays. The number of hours per day would vary depending on the
workload, but I'm estimating it would be at least 6 hours a day.
We have a great crew here. We work hard, but we also have a good time. If
interested, please email me back or call me at the number below.
Laurie Colbert
Huntington Memorial Hospital
(626) 397-8620
------------------------------
Message: 2
Date: Fri, 11 Jun 2004 11:45:56 -0600
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: [Histonet] Frozen tissue to be embedded in OCT later
To: "GUTIERREZ, JUAN" <juan.gutierrez <@t> christushealth.org>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID: <3.0.6.32.20040611114556.00c175c8 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"
Tissues frozen in Liquid nitrogen but NOT embedded in OCT can be embedded
in OCT. Do NOT thaw the tissue, that is not necessary. Just embed your
frozen tissue in cold (refrigerated OCT). Surround tissue as usual, and
then freeze this whole thing. You can actually do this in the cryotat on a
Peltier platform, OR you can sit this embedded tissue block on dry ice.
Immersion in Liquid nitrogen sometimes cracks OCT in our lab, but you may
get away with doing that to have block form rapidly.
You can also immerse block in dry ice/isopentane or hexane slurry - the
same as snap freezing an OCT embedded tissue. The cold OCT does not
appreciably thaw the frozen tissue, and OCT will interface with the frozen
tissue surfaces nicely. Make sure you don't get bubbles.
Juan's suggestion of mounting frozen tissue from -80C freezer directly on a
cryostat block holder with OCT is excellent, you can add more OCT around
tissue if needed for additional support, it works well and does not
appreciably thaw the tissue.
I would avoid thawing tissue if it is destined for cryomicrotomy and keep
it frozen while making a block.
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
------------------------------
Message: 3
Date: Fri, 11 Jun 2004 14:16:37 -0400
From: "Demarinis, Carolyn" <cdemarinis <@t> SARATOGACARE.ORG>
Subject: [Histonet] JCAHO
To: "HISTONET \(E-mail\)" <HISTONET <@t> PATHOLOGY.SWMED.EDU>
Message-ID:
<F15F698E03068A4CA11D19D3E0ED28150FADC6 <@t> shexch1.saratogacare.org>
Content-Type: text/plain; charset="iso-8859-1"
There has been much discussion in our hospital about laboratory staffing
requirements for
Joint Commission (JCAHO).
Is anyone aware of a national benchmark for staffing requirements for
laboratories?
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Message: 4
Date: Fri, 11 Jun 2004 13:41:04 -0500
From: mari.ann.mailhiot <@t> leica-microsystems.com
Subject: Re: [Histonet] (no subject)
To: "Rush, Joyce" <Joyce.Rush <@t> sjmcmn.org>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OFED446D35.ACC0A452-ON86256EB0.005AD82B-86256EB0.00669E63 <@t> e-leica.com>
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Joyce
If you have the luxury of two processors you can process small bx's on one
processor and the larges on another. In the lab I used to work at we
processed small bx's in a separate enclosed processor. The times were 20
minutes each station. Needle bx's have an even shorter processing time of
10 minutes each station. When we processed bx's we were very carefull
about not using heat or vacuum. It will cause the bx's to over harden. Also
make sure your last paraffin is solvent free. Hopefully the processor has
at least three paraffin's stations.
The other thing that can affect processing is having too many 100%
alcohols. You can take the bound water out of the tissue by using too
many. The specimens will be very dry and require a lot of soaking on ice.
Freida Carson recommends no more than two 100% alcohols in her Self
Instructional Text book on Histotechnology. I have found this to be very
true.
If you only have one tissue processor for both large specimens and bx's a
time of no longer than 45 minutes each station is recommended. Some of the
problems that may occur with this type of processing schedule is that the
large specimens may not be fixed long enough. If you have heat on any
stations that heat will affect the small biopsies.
Hope this helps!
Regards
Mari Ann Mailhiot BA HT ASCP
Application Specialist
Leica Technical Assistance Center
800 248 0123 x7267
847 236 3063 fax
mari.ann.mailhiot <@t> leica-microsystems.com
www.leica-microsystems.com
"Rush, Joyce"
<Joyce.Rush <@t> sjmcmn.org> To:
<histonet <@t> lists.utsouthwestern.edu>
Sent by: cc:
histonet-bounces <@t> lists.utsouth Subject:
[Histonet] (no subject)
western.edu
06/11/2004 09:37 AM
I am a Lab Manager, not a Histotech, and I need your help. We are a general
pathology practice and need to know what kind of processing cycles others
use for small bx's. Currently our lab processes all tissues together,
large and small, and therefore has much trouble with over processed bx's.
I would very much appreciate your guidance so that I can help our histology
area move forward.
Thanks so much!
Joyce
Joyce A. Rush, BS, MT(ASCP)
Laboratory Manager
St Joseph's Medical Center
523 North Third Street
Brainerd, MN 56401
Office:218-828-7500 Fax: 218-828-7510
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Brainerd, MN 56401
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------------------------------
Message: 5
Date: Fri, 11 Jun 2004 13:41:02 -0500
From: "Poteete, Jacquie A." <japoteete <@t> saintfrancis.com>
Subject: [Histonet] Histology exam blocks
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<D423BBB85E91D411A38D42003000996506973E8D <@t> mailnt1.saintfrancis.com>
Content-Type: text/plain
Several weeks ago, someone was looking for blocks to use for the Histology
exam. The PAs have the tissue ready for me, but the "gustnado" wiped out
pieces of our computer system. My saved emails were part of the wipe out,
along with several hospital windows and about 12 trees, so please contact me
again if you still need the blocks. Ya gotta love Oklahoma in the Spring!
Jacquie Poteete MT(ASCP)QIHC
Lead Technologist, IHC Laboratory
Saint Francis Hospital, Tulsa, OK
japoteete <@t> saintfrancis.com
********* Email Confidentiality Statement *********
Visit http://www.saintfrancis.com/emailconf.asp
------------------------------
Message: 6
Date: Fri, 11 Jun 2004 11:40:46 -0700
From: Patti Loykasek <ploykasek <@t> phenopath.com>
Subject: FW: [Histonet] cd138 vendor
To: histonet <histonet <@t> pathology.swmed.edu>
Message-ID: <BCEF4ABF.5990%ploykasek <@t> phenopath.com>
Content-Type: text/plain; charset="ISO-8859-1"
------ Forwarded Message
From: Patti Loykasek <ploykasek <@t> phenopath.com>
Date: Fri, 11 Jun 2004 10:14:27 -0700
To: Martha Ward <mward <@t> wfubmc.edu>
Subject: Re: [Histonet] cd138 vendor
Hi Martha. Here¹s the info on our CD138. It has worked well for us with
pretreatment, and a pretty decent titer. Good luck with your search.
Ab: CD138
Supplier: Serotec
Cat#: MCA681H
Clone: B-B4>
Patti Loykasek
PhenoPath Laboratories
Seattle, WA
Can anyone recommend a vendor, etc. for CD138 (syndecan-1) that works
> well on either the Ventana NexEs or the Dako stainer? Thanks in
> advance!
>
> Martha Ward
> Wake Forest University Baptist Medical Center
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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------------------------------
Message: 7
Date: Fri, 11 Jun 2004 15:18:20 -0500
From: "Boone, Linda (HSC)" <Linda-Boone <@t> ouhsc.edu>
Subject: [Histonet] Rabbit Eyes
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<F4D83F1A4443D6459DA7E983AB5B71970CB157E7 <@t> PARADOX.hsc.net.ou.edu>
Content-Type: text/plain; charset="US-ASCII"
Hello All,
I was wondering if anyone out there processes and sections adult rabbit
eyes ? I am new at this and don't know what the optimum fixative is, for
how long and how long of a processing program to use. I will do a search
of the archives as soon as I post this. I am in dire need of any
information or source of information to locate. Thanks in advance. Linda
Boone.
Linda-boone <@t> ouhsc.edu
DMEI
Oklahoma City, OK
------------------------------
Message: 8
Date: Fri, 11 Jun 2004 16:29:49 -0400
From: "DiCarlo, Margaret" <MDiCarlo <@t> KaleidaHealth.Org>
Subject: [Histonet] unsubscribe
To: "'histonet <@t> pathology.swmed.edu'" <histonet <@t> pathology.swmed.edu>
Message-ID:
<DF6AB9298498D31199CF0008C7E6C12005223697 <@t> kalmb02.kaleidahealth.org>
Content-Type: text/plain; charset="iso-8859-1"
Peggy DiCarlo HT (ASCP)
Orthopedics Bone Lab
Buffalo General Hospital
100 High St.
Buffalo, NY 14203
716-859-1293
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Message: 9
Date: Fri, 11 Jun 2004 15:57:26 -0400
From: "Bartlett, Jeanine" <JQB7 <@t> CDC.GOV>
Subject: RE: [Histonet] JCAHO
To: "Demarinis, Carolyn" <cdemarinis <@t> SARATOGACARE.ORG>, "HISTONET
\(E-mail\)" <HISTONET <@t> PATHOLOGY.SWMED.EDU>
Message-ID:
<CB857F6460D42E4AAEA195054A25406C08121B <@t> m-ncid-2.NCID.CDC.GOV>
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Perhaps this will help.
http://www.jcaho.org/accredited+organizations/laboratory+services/standards/
revisions/std_rev_lab.htm
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of
Demarinis, Carolyn
Sent: Fri 6/11/2004 2:16 PM
To: HISTONET (E-mail)
Cc:
Subject: [Histonet] JCAHO
There has been much discussion in our hospital about laboratory
staffing requirements for
Joint Commission (JCAHO).
Is anyone aware of a national benchmark for staffing requirements
for laboratories?
****************************************************************************
***********
CONFIDENTIALITY NOTICE: This e-mail communication and any
attachmentsmay contain confidential and privileged information for
the
use of the designated recipients named above. If you are not the
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If you have received this communication in error, please notify
Saratoga
Hospital immediately by e-mail at privacy <@t> saratogacare.org and
destroy
all copies of this communication and any attachments.
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------------------------------
Message: 10
Date: Fri, 11 Jun 2004 14:36:57 -0700 (PDT)
From: Stacey Burton <staceylburton <@t> yahoo.com>
Subject: [Histonet] cassette labeler
To: Histonet <histonet <@t> pathology.swmed.edu>
Message-ID: <20040611213657.51498.qmail <@t> web14524.mail.yahoo.com>
Content-Type: text/plain; charset=us-ascii
I am looking for a cassette labler. Please, if anyone has any input on good
histology cassette lablelers, let me know.
Thanks,
Stacey Burton, H.T. ASCP
Lab Coordinator
Uropath SA TX
210-521-7700
---------------------------------
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Friends. Fun. Try the all-new Yahoo! Messenger
------------------------------
Message: 11
Date: Fri, 11 Jun 2004 15:20:14 -0700
From: "Tom Wells" <Tom_Wells <@t> bcit.ca>
Subject: [Histonet] best microtomes
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OF0F740113.2897507F-ON88256EB0.007A48AC-88256EB0.007AB463 <@t> bcit.ca>
Content-Type: text/plain; charset=us-ascii
Thanks to everyone who replied to my query about microtomes. Lieca and
Microm seem to be the most popular. I look forward to trying them out.
Tom
------------------------------
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