[Histonet] quantitative iron testing/processor solutions

[Willis, Donna]

Dana,
Are ya'll using Methylene Blue or Cobalt Chloride to dye the tissue blue.
We use Cobalt Chloride (the indicator used in Dririte)and it is a heavy
metal.  We send fresh tissue for our quantitative iron analysis.

Donna Willis
Histology Lab Manager
Harris Methodist Fort Worth, Tx

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Hoops,
Maureen
Sent: Friday, June 18, 2004 9:07 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] quantitative iron testing/processor solutions



Our lab recently had a case sent out for quantitative iron analysis that
yielded abnormal results.  The pathologists requested we submit a sample of
the methylene blue dye we had been using to color our processing alcohols.
Based upon the sample assays, the methylene blue was the cause of the
elevated metal levels. This raises several questions --
Do other laboratories utilize methylene blue coloring in their processor
solutions?
If so, have you ever experienced a similar situation with heavy metal
testing?
Does anyone use Fast Green (Light Green) for a coloring agent in the
alcohols of their processor?  If so, does it interfere with testing?
Does anyone know of an agent we can use to color our alcohols (tissues) that
does not hinder quantitative iron and/or heavy metal analysis?
Thanks for your help
Dana



The information contained in this message and any attachments is intended
only for the use of the individual or entity to which it is addressed, and
may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from
disclosure under applicable law.  If you are not the intended recipient, you
are prohibited from copying, distributing, or using the information.  Please
contact the sender immediately by return e-mail and delete the original
message from your system.

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law.  If you are not the intended recipient, you are prohibited from copying, distributing, or using the information.  Please contact the sender immediately by return e-mail and delete the original message from your system.