[Histonet] RE: Histonet Digest, Vol 7, Issue 22
Breeden, Sara
sbreeden <@t> nmda.nmsu.edu
Fri Jun 18 09:21:19 CDT 2004
To kzhong888 <@t> yahoo.com re Manual for IEC Cryostat
Just coincidentally, I have had to plug in the ol' 3398 this week to do a project here. I located the entire "Installation, Operation, and Service Manual for Minotome Microtome-Cryostat" and you are welcome to have a copy. Contact me at my work email address and give me your mailing address and I'll be happy to send it!
sbreeden <@t> nmda.nmsu.edu
PS this is my first-ever reply to this type of list, so forgive me if I've done it wrong!
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
histonet-request <@t> lists.utsouthwestern.edu
Sent: Friday, June 18, 2004 8:06 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 7, Issue 22
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Today's Topics:
1. Re: Alcian Blue GX8 (Michelle D. Moore)
2. RE: Pneumo stain problems (GUTIERREZ, JUAN)
3. Streck fixed tissue (JENNIFER SCHUMACHER)
4. manual for IEC 3398 or 2488 cryostat microtomes (dfs dsaf)
5. Thank you for you repsonses (Michelle D. Moore)
6. Gms for Pneumo (Cazares, Ruth)
7. Responses to my Alcian Blue 8GX question (kevin williams)
8. RE: kids and histology (Pamela Marcum)
9. RE: OFFTOPIC: Help me make this young man's day!
(Stacy McLaughlin)
10. x-gal staining FFPE (Jenny Oblander)
11. Re: GMS for Pneumo (Gayle Callis)
12. RE: Gms for Pneumo (Cazares, Ruth)
13. Burn Artefact, pneumo immuno (JColCLEFA <@t> aol.com)
14. Re: Neutral mucus stain (Andrew Kennedy)
15. RE: kids and histology (Patsy Ruegg)
16. Re: kids and histology (Linda Jenkins)
17. I'm sorry I can not reply immediately because I am out of the
office, returning on Tuesday June 22, (John Ryan)
18. To Dr. Manal Galal, Cairo, Egypt. (George Cole)
19. Re: kids and histology (SMITH,REBEKAH FELICIA)
20. Help Please! Sentinel Lymph Nodes! (Gladney, Diane C Ms MACH)
21. Re: kids and histology (Margaret Horne)
22. p. carinii stain (TERRI BRAUD)
23. RE: kids and histology (Barry R Rittman)
24. RE: kids and histology
(Marshall Terry Dr, Consultant Histopathologist)
25. RE: kids and histology (Barry R Rittman)
26. RE: kids and histology
(Marshall Terry Dr, Consultant Histopathologist)
----------------------------------------------------------------------
Message: 1
Date: Thu, 17 Jun 2004 08:54:28 -0600
From: "Michelle D. Moore" <tmhpath <@t> amigo.net>
Subject: Re: [Histonet] Alcian Blue GX8
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <005d01c4547a$fff4e7c0$4000a8c0 <@t> ever>
Content-Type: text/plain; charset="iso-8859-1"
Kevin,
I purchased my last two bottles of Alcian Blue GX8 from Cardinal Health
formerly Allegience for $47.34/bottle catalog# S7442-3, phone #
800-964-5227. Lots of luck to you, my understanding is that the supply of
Alcian Blue is running out. Anatech has found a way to make their own Alcian
Blue that is more stable and produces better results according to one of the
people I've talked to at the company, but I'm in a small hospital where cost
is of great importance and I have not had any complaints about my staining
and I use this stain a lot. Hope this helps.
Michelle Moore HT(ASCP)
TMH
Craig, CO
----- Original Message -----
From: "kevin williams" <akwilliams75 <@t> hotmail.com>
To: <histonet <@t> pathology.swmed.edu>
Sent: Wednesday, June 16, 2004 12:58 PM
Subject: [Histonet] Alcian Blue GX8
>
> Dear All:
> I am looking for Alcian Blue, but the price seems to have gone like
> the oil price, can anyone tell me of a price less than $225.
> Thanks for your help.
> _________________________________________________________________
>
> [1]Looking to buy a house? Get informed with the Home Buying Guide
> from MSN House & Home.
>
> References
>
> 1. http://g.msn.com/8HMBENUS/2746??PS=47575
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> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 2
Date: Thu, 17 Jun 2004 12:16:57 -0500
From: "GUTIERREZ, JUAN" <juan.gutierrez <@t> christushealth.org>
Subject: RE: [Histonet] Pneumo stain problems
To: "Cazares, Ruth" <RCazares <@t> schosp.org>,
<histonet <@t> pathology.swmed.edu>
Message-ID:
<A61F23E937E4DA488E0C0F60093843D90F6A43 <@t> ccetxm030.echristus.net>
Content-Type: text/plain; charset="iso-8859-1"
We are doing both. The immuno is so much nicer. We use Ventana's pneumo since we have Benchmarks, but I believe they get theirs from Cell Marque.
Ventana Med. Sys. 1-800-227-2155
Cell Marque 1-800-665-7284
Juan C. Gutierrez, HT(ASCP)
Histology Laboratory Supervisor
Christus Santa Rosa Hospital
333 N. Santa Rosa Ave.
San Antonio, TX 78207
(210)704-2533
-----Original Message-----
From: Cazares, Ruth [mailto:RCazares <@t> schosp.org]
Sent: Thursday, June 17, 2004 11:55 AM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] Pneumo stain problems
Hello again histonetters,
I have been having problems with the GMS stain for p. Carinii. It seems
that some days the stain is absolutely beautiful and other days (using the
same proven control ) it does not work. We have made up all new reagents to
no avail. I am now considering changing to Immuno staining for Pneumo and
would like some feedback from those of you who are doing this, pros and
cons. Any and all info will be greatly appreciated.
Thank You,
Ruth Cazares
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Message: 3
Date: Thu, 17 Jun 2004 12:26:18 -0500
From: "JENNIFER SCHUMACHER" <JSCHUMA1 <@t> FAIRVIEW.ORG>
Subject: [Histonet] Streck fixed tissue
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s0d18df1.069 <@t> gw-northdom.fairview.org>
Content-Type: text/plain; charset=US-ASCII
Does anyone stain trichromes differently for Streck fixed tissue?
Thanks in Advance for any suggestions. Jen
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------------------------------
Message: 4
Date: Thu, 17 Jun 2004 11:00:53 -0700 (PDT)
From: dfs dsaf <kzhong888 <@t> yahoo.com>
Subject: [Histonet] manual for IEC 3398 or 2488 cryostat microtomes
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20040617180053.39794.qmail <@t> web41604.mail.yahoo.com>
Content-Type: text/plain; charset=us-ascii
hi histonetters
does any one have a old copy of service or operation manual for IEC cryostat microtome model 3398 or 2488. I desperately need a copy and sould be happy to pay for copy and postage expenses.
Thanks kirk KZhong888 <@t> yahoo.com
---------------------------------
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------------------------------
Message: 5
Date: Thu, 17 Jun 2004 08:57:57 -0600
From: "Michelle D. Moore" <tmhpath <@t> amigo.net>
Subject: [Histonet] Thank you for you repsonses
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <006601c4547b$7a658280$4000a8c0 <@t> ever>
Content-Type: text/plain; charset="iso-8859-1"
I would like to thank those of you who responded to my request for help with the tissue exemption policies. It helped my a lot and will give me more ammunition to show them what other places, even smaller places are doing. Thank you again that's why I love this forum, it's very helpful. Have a great weekend everyone.
Michelle Moore HT(ASCP)
TMH
Craig, CO
------------------------------
Message: 6
Date: Thu, 17 Jun 2004 13:27:13 -0500
From: "Cazares, Ruth" <RCazares <@t> schosp.org>
Subject: [Histonet] Gms for Pneumo
To: histonet <@t> pathology.swmed.edu
Message-ID:
<229A3566B9F0D311826E00D0B7441D79073C456B <@t> swedish_nt1.schosp.org>
Content-Type: text/plain
In Response to Gayle's Inquiry as to how we do our stain,
We use the GMS procedure in Freida Carson's book, but we use the microwave:
Heat the chromic acid @ 80% power for 50 seconds
Put the slides into the hot chromic and let sit for 10 minutes.
Wash and put in bisulfite for I minute and rinse.
Heat silver (25ml stock methenamine silver, 25ml DI water and 2 ml 5% Borax)
37 seconds on high
Place slides into hot silver and microwave on high for 10 seconds, let sit
for 3-5 minutes checking
Under the microscope after 3 minutes.
Rinse, tone with 0.2% gold, then 2% thiosulfate and counterstain with fast
green.
The fungus comes out very well each time, but the pneumo is very
inconsistent.
Ruth Cazares
*** Confidentiality Statement ***
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which it is addressed and may contain information that is privileged and
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Message: 7
Date: Thu, 17 Jun 2004 18:44:42 +0000
From: "kevin williams" <akwilliams75 <@t> hotmail.com>
Subject: [Histonet] Responses to my Alcian Blue 8GX question
To: histonet <@t> pathology.swmed.edu
Message-ID: <BAY18-F53Ejxq6pgtq300019419 <@t> hotmail.com>
Content-Type: text/plain
Sigma has a 10 gram bottle for $94.10. Though it is still up there with
the price of milk. Their site is sigma-aldrich.com
We're Raymond A Lamb Ltd/UK and we currently hold stock of Cat Ref S6-2
Alcian Blue 8GX (Ingrain Blue I) in the UK @ GBP £75.00/25g or available
(lead time approx. a couple of weeks) through our USA office @ $145.00/25g
T 919 387 1237 F 919 387 1736.
See our website www.ralamb.com
Kind regards.
Sarah Lamb Hughes
RAYMOND A LAMB LTD/UK
T +44 1323 737000 F +44 1323 733000
The last time I looked at Anatech's website (www.anatechltdusa.com), they
were offering alcian blue for a little under $100 for a 25 gram jar.
Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University
School of Graduate Medical Sciences
Podiatric Medicine and Surgery
Miami Shores, Florida
Dear Sir,
We are one research & development based custom manufacturer in China,
in line of Biochemicals, Specialty Chemicals.
We can supply you Alcian Blue 8GX, BSC certified, please advise us the
quantity you need.
Kind Regards.
-
Minggeng Wang, Ph.D / President
SUZHOU SINOERA CHEM CO., LTD.
No.5508, 125 Binhe Road
Suzhou New & Hi-Tech District
215011 China
Fax: +86 512 68224995
Tel: +86 1380 6217039
+86 512 68246939
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------------------------------
Message: 8
Date: Thu, 17 Jun 2004 14:49:52 -0400
From: "Pamela Marcum" <pmarcum <@t> polysciences.com>
Subject: RE: [Histonet] kids and histology
To: "Tom McNemar" <TMcNemar <@t> lmhealth.org>, "'MARY T HODGES'"
<hodges420 <@t> msn.com>, "HistoNet Server" <histonet <@t> pathology.swmed.edu>
Message-ID: <001301c4549b$dfb5e380$4000a8c0 <@t> PMARCUM2K>
Content-Type: text/plain; charset="iso-8859-1"
Sorry to tag on here but I have a question.
I am sorry to bother you all with this questions however someone just
called
asked me how to do a Trap Stain on bone and I do not have a protocol to
give
them. She currently does not have access to a computer to ask herself
so
we can do one of two things. Please either send me the protocol by
e-mail
or fax it to 800-949-3291 to my attention or let me know you have one
for
her and I will send your her fax number for a direct contact. I know we
have has this on HistoNet many times and I just never copied the
information
for future use.
Thanks,
Pam Marcum
Thanks,
Pam Marcum
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Tom
> McNemar
> Sent: Thursday, June 17, 2004 10:00 AM
> To: 'MARY T HODGES'; HistoNet Server
> Subject: RE: [Histonet] kids and histology
>
>
> For one thing, we take a double headed scope and some slides. The kids
> really seem to enjoy looking at tonsils, brain, etc. I have some
> specimens
> (gallstones, appendix, lung, etc) encased in plastic resin and they enjoy
> that. The National Society for Histotechnology has a wonderful pamphlet
> about histotechnology as a career. It can be ordered from their
> website for
> shippng charges only (I think it's $8 per 25 pamphlets).
>
> Tom.
>
> -----Original Message-----
> From: MARY T HODGES [mailto:hodges420 <@t> msn.com]
> Sent: Wednesday, June 16, 2004 9:51 PM
> To: HistoNet Server
> Subject: [Histonet] kids and histology
>
>
> Good evening all,
> Does any one have some ideas what kids today would respond to at a job
> awareness fair. something comic but maybe fun about our jobs. Any input
> appreciated. Thanks, Tere Hodges St. Mary's Hospital Tucson, Az,
> _______________________________________________
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> Histonet <@t> lists.utsouthwestern.edu
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------------------------------
Message: 9
Date: Thu, 17 Jun 2004 14:57:46 -0400
From: Stacy McLaughlin <Stacy_McLaughlin <@t> cooley-dickinson.org>
Subject: RE: [Histonet] OFFTOPIC: Help me make this young man's day!
To: "'A. Erickson'" <andrae <@t> u.washington.edu>, Vinnie Della Speranza
<dellav <@t> musc.edu>
Cc: histonet <@t> pathology.swmed.edu
Message-ID: <3D502BBF5356D31184650090275B750D0346C7B8 <@t> MAIL>
Content-Type: text/plain
Dear Vinnie and Histonetters,
As a military wife and a veteran myself, I thank you for your concern and
support for those serving our country overseas.
It's so easy for all of us to get caught up in our day to day lives. Let us
never forget those who are out there making sacrifices so that we may all
have our pursuit of happiness.
Best Regards,
Stacy
-----Original Message-----
From: A. Erickson [mailto:andrae <@t> u.washington.edu]
Sent: Thursday, June 17, 2004 11:41 AM
To: Vinnie Della Speranza
Cc: histonet <@t> pathology.swmed.edu
Subject: Re: [Histonet] OFFTOPIC: Help me make this young man's day!
Dear Vinnie,
An excellent idea1 I'll add these two young men to the seven I'm
writing & sending care packages to. the husband of a friend of mine is in
bagdad (he'sa Nat'l Gard medic) and we send stuff to his unit. You would be
surprised at the number of our guys who dont get mail from home. It's all
appreciated. Regards, andra erickson, WaNPRC Seattle Wa.
On Wed, 16 Jun 2004, Vinnie Della Speranza wrote:
> I hope that all of you will indulge me this off topic post. I'm
> willing to endure the flames if even just a few of you will consider
> this worthy of your effort
>
> a coworker's son is a marine serving in Baghdad. He told his mom about
> a buddy in his outfit who doesn't get any mail from home. I told her
> that I would be proud to write him. For all that he is sacrificing for
> us, it seems wrong that he should feel unappreciated.
>
> as soon as I complete this post I will be drafting a letter thanking
> him for his sacrifice on my behalf to preserve freedom. These men and
> women are putting everything on the line for us in a country filled
> with individuals who do not seem to value life
>
> I'm sharing this with you because I thought that some of you might
> want to drop these boys a line and let them know that they are not
> forgotten and how proud we are of them.
>
> the young man who doesn't receive mail is
>
> Corporal Shawn Sanders
> MWSS-273 Eng. Division
> Unit 78555
> FPO-AP 09509-8555
>
> My coworker's son is Sargeant Brandon Bridges in the same unit if you
> get the urge to write them both.
>
> it would be so cool if these guys could receive a sack of mail from
> back home. Just think of the message it would send to their entire
> unit!
>
> thanks for listening!!
>
>
> Vinnie Della Speranza
> Manager for Anatomic Pathology Services
> Medical University of South Carolina
> 165 Ashley Avenue Suite 309
> Charleston, SC 29425
> Ph: 843-792-6353
> fax: 843-792-8974
>
> _______________________________________________
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> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
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Message: 10
Date: Thu, 17 Jun 2004 15:16:50 -0500
From: Jenny Oblander <Jenny-Oblander <@t> omrf.ouhsc.edu>
Subject: [Histonet] x-gal staining FFPE
To: "Histonet (E-mail)" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<B9D4D13500D6D411B4E70002B31B5E6C031FADA3 <@t> mail.omrf.ouhsc.edu>
Content-Type: text/plain; charset="iso-8859-1"
Hi Everyone,
I need your help. My boss wants to do a x-gal on FFPE sections. I
have tried to find a protocol on the web but I can't find anything but
cryosection staining or whole mount. My question is ...Can you stain a FFPE
slide with x-gal? And if you can, how? Thanks Jenny
J.Oblander, HT (A.S.C.P.)
Comparative Medicine
Oklahoma Medical Research Foundation MS#32
825 NE 13th St.
Okc,Ok 73104
jenny-oblander <@t> omrf.ouhsc.edu
405-271-7083
------------------------------
Message: 11
Date: Thu, 17 Jun 2004 14:20:27 -0600
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: [Histonet] Re: GMS for Pneumo
To: "Cazares, Ruth" <RCazares <@t> schosp.org>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID: <3.0.6.32.20040617142027.00c02400 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"
Have you ever not done this with a MW, we do classic, old fashioned purist
long GMS without MW and Pneumocystis works without problems, in fact is
very easy to stain. One of our research groups studies this organism in
lungs. Try the method the old fashioned purist way and see what happens.
Could it be that hot chromic acid is over-oxidizing your Pneumo cell wall
resulting in inconsistent results. This step could be done at room
temperature - it doesn't add that much time to protocol.
When we did use a MW, we agitated/stirred MW heated solutions BEFORE
immersing sections, to make sure temperature is evenly dispersed over
section/slide. A tongue depressor or plastic Pasteur transfer pipette works
nicely.
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
------------------------------
Message: 12
Date: Thu, 17 Jun 2004 15:24:58 -0500
From: "Cazares, Ruth" <RCazares <@t> schosp.org>
Subject: RE: [Histonet] Gms for Pneumo
To: histonet <@t> pathology.swmed.edu
Message-ID:
<229A3566B9F0D311826E00D0B7441D79073C458B <@t> swedish_nt1.schosp.org>
Content-Type: text/plain
We use 2 different controls, one for fungus and one for pneumo. We always
leave the pneumo in the silver longer but sometimes it just gets brown-black
all over and the organisms don't stand out. And then a couple days later we
do it (the same way) and it is absolutely beautiful (text book quality). I
can't figure out what makes it so variable. Do the cut control slides get
old and not stain? I just don't know.
Ruth Cazares
-----Original Message-----
From: Jones, Sarah - RAS [mailto:sjones <@t> rallansci.com]
Sent: Thursday, June 17, 2004 2:56 PM
To: 'Cazares, Ruth'
Subject: RE: [Histonet] Gms for Pneumo
Ruth,
Are you using a regular Fungus Control Slide or a Pneumocystis Control
Slide? Pneumocystis always takes longer to stain, so a Pneumocystis Control
Slide should be used.
Sarah
-----Original Message-----
From: Cazares, Ruth [mailto:RCazares <@t> schosp.org]
Sent: Thursday, June 17, 2004 2:27 PM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] Gms for Pneumo
In Response to Gayle's Inquiry as to how we do our stain,
We use the GMS procedure in Freida Carson's book, but we use the microwave:
Heat the chromic acid @ 80% power for 50 seconds
Put the slides into the hot chromic and let sit for 10 minutes.
Wash and put in bisulfite for I minute and rinse.
Heat silver (25ml stock methenamine silver, 25ml DI water and 2 ml 5% Borax)
37 seconds on high
Place slides into hot silver and microwave on high for 10 seconds, let sit
for 3-5 minutes checking
Under the microscope after 3 minutes.
Rinse, tone with 0.2% gold, then 2% thiosulfate and counterstain with fast
green.
The fungus comes out very well each time, but the pneumo is very
inconsistent.
Ruth Cazares
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Thank you for your cooperation.
*************************
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------------------------------
Message: 13
Date: Thu, 17 Jun 2004 17:21:10 EDT
From: JColCLEFA <@t> aol.com
Subject: [Histonet] Burn Artefact, pneumo immuno
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4c.2d7cee00.2e036546 <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"
Might your pathologist be speaking of cautery artefact? We get specimens
every once in a while which have a cauterized margin- the tissue is literally
burned- and there is nothing I know to do to reclaim this tissue.
We use the pneumocystis immuno in our lab but mostly as a confirmation (our
GMS is reliable since it's been tweaked to death) and also if there are
trophozoite forms of the organism suspected. It seems pretty reliable but does not
highlight the capsule of the bug as well as the GMS does, so the pathologists
need to realise the traditional look of the organisms will not be as obvious.
Questions? e-me JC (QIHC)
------------------------------
Message: 14
Date: Fri, 18 Jun 2004 07:25:19 +1000
From: "Andrew Kennedy" <kennedya <@t> email.cs.nsw.gov.au>
Subject: [Histonet] Re: Neutral mucus stain
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <20040618072210.SM01316 <@t> crgcsls813>
Content-Type: text/plain; charset="us-ascii"
Hi Nancy,
Is this stuff still positive in a Diastase PAS?
Might it be a glycoprotein or a glycolipid?
A High Iron Diamine stain will distinguish glycoproteins from other
mucosubstances.
Try a Sudan fat stain or an Oil Red O for glycolipids
Regards
Andrew Kennedy
Senior Science Officer
Anatomical Pathology
Concord Repatriation General Hospital
Hospital Road
Concord NSW 2139
ph: +612 9767 6115
Fax +612 9767 8427
"corpora non agunt nisi fixata"
Message: 10
Date: Tue, 15 Jun 2004 21:27:13 -0700
From: staub <@t> gonzaga.edu
Subject: [Histonet] neutral mucus stain?
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <1087360033.40cfcc2153413 <@t> zagmail.gonzaga.edu>
Content-Type: text/plain; charset=ISO-8859-1
Does anyone know of a diagnostic stain for neutral mucus? OR does anyone
know a good control tissue we could use? We have identified a PAS +, alcian
blue negative, toluidine blue orthochromatic, carmine red slight positive
(and we know it's not glycogen) exocrine gland secretion (in an amphibian)
and think it is neutral mucus. IS there anything else we can try that isn't
redundant?
thank you much.
Nancy Staub
Gonzaga University, Spokane, WA
------------------------------
Message: 15
Date: Thu, 17 Jun 2004 15:52:19 -0600
From: "Patsy Ruegg" <pruegg <@t> colobio.com>
Subject: RE: [Histonet] kids and histology
To: "Pamela Marcum" <pmarcum <@t> polysciences.com>, "Tom McNemar"
<TMcNemar <@t> lmhealth.org>, "'MARY T HODGES'" <hodges420 <@t> msn.com>,
"HistoNet Server" <histonet <@t> pathology.swmed.edu>
Message-ID: <NGBBIMHHKLGNCOCPDKBOGEPECHAA.pruegg <@t> colobio.com>
Content-Type: text/plain; charset="iso-8859-1"
Pam,
Sigma sells a kit for doing TRAP. It is traditionally done on blood or
marrow smears for hematopathology using the Sigma Kit. Hairy cell leukemia
cells are tartrate resistant acid phos positive. Osteoclasts and some T
lymphocytes are also TRAP+. I adapted this Sigma TRAP kit to stain
osteoclasts in bone in samples processed without decalcification in GMA. I
mixed the kit components and placed the sections in the reagent in a 37dc
oven for 1 hr. This is an enzyme histochemical stain and will stain the
granules in multinucleated osteoclast cells red. We used a light green
counter stain with this. There are reports of people using this enzyme
method sucessfully on ffpe bone if the fixation is not too long, I have not
done this myself. Also, Hermina does this on EDTA decalcified bone. There
is also an anti-TRAP antibody method for IHC that came out a few years ago,
it was written up in JOH, I have not tried it.
Patsy
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Pamela
Marcum
Sent: Thursday, June 17, 2004 12:50 PM
To: Tom McNemar; 'MARY T HODGES'; HistoNet Server
Subject: RE: [Histonet] kids and histology
Sorry to tag on here but I have a question.
I am sorry to bother you all with this questions however someone just
called
asked me how to do a Trap Stain on bone and I do not have a protocol to
give
them. She currently does not have access to a computer to ask herself
so
we can do one of two things. Please either send me the protocol by
e-mail
or fax it to 800-949-3291 to my attention or let me know you have one
for
her and I will send your her fax number for a direct contact. I know we
have has this on HistoNet many times and I just never copied the
information
for future use.
Thanks,
Pam Marcum
Thanks,
Pam Marcum
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Tom
> McNemar
> Sent: Thursday, June 17, 2004 10:00 AM
> To: 'MARY T HODGES'; HistoNet Server
> Subject: RE: [Histonet] kids and histology
>
>
> For one thing, we take a double headed scope and some slides. The kids
> really seem to enjoy looking at tonsils, brain, etc. I have some
> specimens
> (gallstones, appendix, lung, etc) encased in plastic resin and they enjoy
> that. The National Society for Histotechnology has a wonderful pamphlet
> about histotechnology as a career. It can be ordered from their
> website for
> shippng charges only (I think it's $8 per 25 pamphlets).
>
> Tom.
>
> -----Original Message-----
> From: MARY T HODGES [mailto:hodges420 <@t> msn.com]
> Sent: Wednesday, June 16, 2004 9:51 PM
> To: HistoNet Server
> Subject: [Histonet] kids and histology
>
>
> Good evening all,
> Does any one have some ideas what kids today would respond to at a job
> awareness fair. something comic but maybe fun about our jobs. Any input
> appreciated. Thanks, Tere Hodges St. Mary's Hospital Tucson, Az,
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 16
Date: Thu, 17 Jun 2004 18:28:20 -0400
From: Linda Jenkins <jlinda <@t> ces.clemson.edu>
Subject: Re: [Histonet] kids and histology
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<5.2.1.1.0.20040617181210.032d3258 <@t> mailhost.ces.clemson.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Dear Tere,
I just finished doing tours for 80 eight grade girls to spark an
interest in science and engineering. I found that almost all the girls
watched a program called C.S.I. (Crime Scene Investigation). This program
is quite the in thing with young people. Capitalizing on that show, I
proceeded to take some cantaloupe "fixed" in ethanol and tell the girls
that the lab had received a mysterious specimen from our university mascot
(a Tiger...orange parts, you see) and we needed to find out what had
happened to our Tiger. The girls drew a number out of the hat and the
winner got to embed a piece of the Tiger and section an already embedded
portion of Tiger/cantaloupe (closely supervised by me...of course). I also
had embedded a goodly number of "tiger" tissue so they each got to take a
piece of the Tiger home with them.
Of course, this would be for middle school and up. There are all
sorts of non biohazardous fruits and veggies that work quite well for show
and tell. All you need is your imagination! Now I'm working on trying to
stage a mock crime scene where we find some tissue and have to rush it
through the lab.
The response has been an overwhelming success!
Have fun!
Linda
Linda Jenkins, HT
Clemson University
Dept. of Bioengineering
Clemson, SC 29634-0905
864.656.5553
http://www.ces.clemson.edu/bio/research/histo/histo.htm
------------------------------
Message: 17
Date: Thu, 17 Jun 2004 19:24:24 -0500
From: "John Ryan" <jryan <@t> sleh.com>
Subject: [Histonet] I'm sorry I can not reply immediately because I am
out of the office, returning on Tuesday June 22,
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s0d1efed.068 <@t> ms1.sleh.com>
Content-Type: text/plain; charset=US-ASCII
I'm sorry I can not reply immediately because I am out of the office, returning on Tuesday June 22, 2004.
------------------------------
Message: 18
Date: Thu, 17 Jun 2004 19:34:48 -0700
From: "George Cole" <georgecole <@t> ev1.net>
Subject: [Histonet] To Dr. Manal Galal, Cairo, Egypt.
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000001c454dc$d3ad8840$084dbad0 <@t> hppav>
Content-Type: text/plain; charset="us-ascii"
Dr. Galal,
Like the first packet, the second packet of CD and pages on muscle and
nerve biopsy technique sent to you has been returned UNCLAIMED.
georgecole <@t> ev1.net
------------------------------
Message: 19
Date: Thu, 17 Jun 2004 22:37:38 -0400 (EDT)
From: "SMITH,REBEKAH FELICIA" <rockbeki <@t> ufl.edu>
Subject: Re: [Histonet] kids and histology
To: CrochiereSteve <@t> aol.com, hodges420 <@t> msn.com,
histonet <@t> pathology.swmed.edu
Message-ID: <1151525936.1087526258719.JavaMail.osg <@t> spnode15>
Content-Type: text/plain; format=flowed; charset=us-ascii
I don't know if this would be feasible in a job fair, but I
remember in grade school we did a science project involving
putting our cheek cells on slides and comparing them to onion
sections on slides. I thought it was fun at least. :-)
--
SMITH,REBEKAH FELICIA
"You are a child of the universe, no less than the trees and the
stars
You have a right to be here and whether or not it is clear to you,
no doubt the universe is unfolding as it should. Therefore be at
peace with G-d, whatever you conceive Him to be. And whatever your
labors and aspirations,in the noisy confusion of life, keep peace
in your soul.-Max Ehrmann,"Desiderata"
------------------------------
Message: 20
Date: Fri, 18 Jun 2004 06:10:43 -0400
From: "Gladney, Diane C Ms MACH" <Diane.Gladney <@t> se.amedd.army.mil>
Subject: [Histonet] Help Please! Sentinel Lymph Nodes!
To: 'HistoNet Server' <histonet <@t> pathology.swmed.edu>
Message-ID: <9D41AB7C56F8304F98537ABD87B249F6626210 <@t> DASMTHGBZ001>
Content-Type: text/plain; charset="iso-8859-1"
Histonetters,
I am desperate need of help with protocols for handling Sentinel Lymph
Nodes. Our pathologist agreed to accept a Sentinel Lymph Node today from
Surgery. We have never done these types of biopsies here before. We do not
have any protocols, safety measures, etc. in place. This was dumped in my
lap very late yesterday and I am trying to jump through hoops to try and
handle this specimen properly. I am trying to get some guidance from
Radiology but I haven't gotten any help from them so far. Does anyone have a
protocol with proper storage and handling of these types of biopsies that
they would be willing to share with me?
If anyone can help me, please either email me your protocols or Fax them to
me at: 803-751-7829.
I need this ASAP. I thank you all for any assistance.
Thanks,
Diane
Diane C. Gladney, HT (ASCP)
Histology /Cytology Supervisor
Moncrief Army Community Hospital
P.O. BOX 484
4500 Stuart Ave.
FT. Jackson, SC 29207
(803) 751-2530
DSN 734-2530
EMAIL: diane.gladney <@t> se.amedd.army.mil OR
dcgx1 <@t> aol.com
------------------------------
Message: 21
Date: Fri, 18 Jun 2004 09:14:58 ADT
From: "Margaret Horne" <mhorne <@t> upei.ca>
Subject: [Histonet] Re: kids and histology
To: histonet <@t> pathology.swmed.edu
Message-ID: <40D2B291.30960.434A1C <@t> localhost>
Content-Type: text/plain; charset=US-ASCII
We hold Grade 6,7,and 8 veterinary camps for kids every summer
and I help to run the histology section. We teach them how to use
a microscope , then how to ID the WBC's and the dif between fish
and mammalian RBC's( 1 to 1 1/2 hour session.) In the second
session , we give them three " cases" to solve. One is a case of
heart worm in a dog, and another a case of leukemia in a cow.
These are slides I borrow from Pathology every year. The final case
is a CSI type. The police have a suspect in a murder who swears
the blood stains on the axe are from the fish he cleaned and ate.
The kids have to answer the question for the police " Is this human
or fish blood?". It's fish actually.
We have about 30 kids is these sessions and I have three or
four people( vet students and retired prof's) helping me as each
child has their own microscope to use ( vet students
classroom/lab) and time is tight. One could adapt this to
multiheaded scopes or scopes that are projected on a screen.
If you want my notes and diagrams , just give me your fax
number.
We always have fun with this,
Margaret
Margaret Horne ,
Histology Teaching Assistant,
Dept. of B.SC.,
Atlantic Veterinary College, U.P.E.I.,
550 University Ave., Charlottetown,
P.E.I., C1A 4P3
Canada
------------------------------
Message: 22
Date: Fri, 18 Jun 2004 08:53:29 -0400
From: "TERRI BRAUD" <terribraud <@t> msn.com>
Subject: [Histonet] p. carinii stain
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <BAY11-F27TWisFPkms500011218 <@t> hotmail.com>
Content-Type: text/plain
I've used both IHC and the traditional GMS stain and greatly prefer
the IHC over GMS. However, when I moved to the University of
Virginia, they were using a wonderful silver stain developed by
Churkian and Schenk. (Lab. Med. 17:87-90, 1986; J. Histotechnnol.
11:19-21, 1988) It oxidizes in Periodic Acid instead of Chromic Acid
and uses a stabilized Ammoniacal Silver. Performed in the microwave
in about 10 minutes for the total stain, it is one of the cleanest,
most consistent silver fungus stains I have seen, and you can't beat
the turn around time! Give this one a try before giving up on silver
fungus stains.
Terri L. Braud, HT(ASCP), Manager
Surgical Pathology Department, OMS Rm 4765
University of Virginia Health System
PO BOX 800214
Charlottesville, VA 22908-0214
Ph. (434) 924-2056, Fax. (434) 924-0217
------------------------------
Message: 23
Date: Fri, 18 Jun 2004 08:29:15 -0500
From: "Barry R Rittman" <Barry.R.Rittman <@t> uth.tmc.edu>
Subject: RE: [Histonet] kids and histology
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<566FB0B522443D43AF02D2ADBE35A6F077FE42 <@t> UTHEVS3.mail.uthouston.edu>
Content-Type: text/plain; charset="us-ascii"
Rebekah
I would also recommend these, at least as a first step.
It has the advantage of being simple and rapid.
We used Q-tips to rub the inside of the cheek, smeared this on a slide.
Either fix with alcohol or just dry. Stain with dilute toluidine blue
for a minutes or so, rinse and dry. While it is preferable to use
immersion oil, a lot of details can be seen just using dried sections.
Kids can see their own cells, many with nuclei and also bacteria on the
surface of the cells.
All you have to do is explain the kid's blue finger tips to the parents.
If you want to explain cell division, we used to show them sections of
onion root tips stained with either Heidenhain's iron hematoxylin or
Feulgen.
Barry
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
SMITH,REBEKAH FELICIA
Sent: Thursday, June 17, 2004 9:38 PM
To: CrochiereSteve <@t> aol.com; hodges420 <@t> msn.com;
histonet <@t> pathology.swmed.edu
Subject: Re: [Histonet] kids and histology
I don't know if this would be feasible in a job fair, but I
remember in grade school we did a science project involving
putting our cheek cells on slides and comparing them to onion
sections on slides. I thought it was fun at least. :-)
--
SMITH,REBEKAH FELICIA
"You are a child of the universe, no less than the trees and the
stars
You have a right to be here and whether or not it is clear to you,
no doubt the universe is unfolding as it should. Therefore be at
peace with G-d, whatever you conceive Him to be. And whatever your
labors and aspirations,in the noisy confusion of life, keep peace
in your soul.-Max Ehrmann,"Desiderata"
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 24
Date: Fri, 18 Jun 2004 14:34:38 +0100
From: "Marshall Terry Dr, Consultant Histopathologist"
<Terry.Marshall <@t> rothgen.nhs.uk>
Subject: RE: [Histonet] kids and histology
To: "Barry R Rittman" <Barry.R.Rittman <@t> uth.tmc.edu>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<FE2DB935F8BBB546B8A1BBF3459C5A1F02DC9E71 <@t> LIL.xRothGen.nhs.uk>
Content-Type: text/plain; charset="iso-8859-1"
I've always wondered, "why onion?".
Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
Consultant Pathologist
Rotherham General Hospital
South Yorkshire
England
terry.marshall <@t> rothgen.nhs.uk
-----Original Message-----
From: Barry R Rittman [mailto:Barry.R.Rittman <@t> uth.tmc.edu]
Sent: 18 June 2004 14:29
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] kids and histology
Rebekah
I would also recommend these, at least as a first step.
It has the advantage of being simple and rapid.
We used Q-tips to rub the inside of the cheek, smeared this on a slide.
Either fix with alcohol or just dry. Stain with dilute toluidine blue
for a minutes or so, rinse and dry. While it is preferable to use
immersion oil, a lot of details can be seen just using dried sections.
Kids can see their own cells, many with nuclei and also bacteria on the
surface of the cells.
All you have to do is explain the kid's blue finger tips to the parents.
If you want to explain cell division, we used to show them sections of
onion root tips stained with either Heidenhain's iron hematoxylin or
Feulgen.
Barry
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
SMITH,REBEKAH FELICIA
Sent: Thursday, June 17, 2004 9:38 PM
To: CrochiereSteve <@t> aol.com; hodges420 <@t> msn.com;
histonet <@t> pathology.swmed.edu
Subject: Re: [Histonet] kids and histology
I don't know if this would be feasible in a job fair, but I
remember in grade school we did a science project involving
putting our cheek cells on slides and comparing them to onion
sections on slides. I thought it was fun at least. :-)
--
SMITH,REBEKAH FELICIA
"You are a child of the universe, no less than the trees and the
stars
You have a right to be here and whether or not it is clear to you,
no doubt the universe is unfolding as it should. Therefore be at
peace with G-d, whatever you conceive Him to be. And whatever your
labors and aspirations,in the noisy confusion of life, keep peace
in your soul.-Max Ehrmann,"Desiderata"
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 25
Date: Fri, 18 Jun 2004 08:43:42 -0500
From: "Barry R Rittman" <Barry.R.Rittman <@t> uth.tmc.edu>
Subject: RE: [Histonet] kids and histology
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<566FB0B522443D43AF02D2ADBE35A6F0635AC0 <@t> UTHEVS3.mail.uthouston.edu>
Content-Type: text/plain; charset="us-ascii"
Terry
It is trivia like this that my wife says that I worry about too much!!
Can use any root from any fast growing plant but onion is used because
it is continuing tradition and if you leave onions in the pantry too
long they will develop roots. Using a bulb, it is easy to place the bulb
suspended on the opening of a jar filled with water and harvest growing
roots as needed.
Perhaps the supply of onion root tips could be the start of a new
cottage industry?
Barry
-----Original Message-----
From: Marshall Terry Dr, Consultant Histopathologist
[mailto:Terry.Marshall <@t> rothgen.nhs.uk]
Sent: Friday, June 18, 2004 8:35 AM
To: Barry R Rittman; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] kids and histology
I've always wondered, "why onion?".
Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant
Pathologist Rotherham General Hospital South Yorkshire England
terry.marshall <@t> rothgen.nhs.uk
-----Original Message-----
From: Barry R Rittman [mailto:Barry.R.Rittman <@t> uth.tmc.edu]
Sent: 18 June 2004 14:29
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] kids and histology
Rebekah
I would also recommend these, at least as a first step.
It has the advantage of being simple and rapid.
We used Q-tips to rub the inside of the cheek, smeared this on a slide.
Either fix with alcohol or just dry. Stain with dilute toluidine blue
for a minutes or so, rinse and dry. While it is preferable to use
immersion oil, a lot of details can be seen just using dried sections.
Kids can see their own cells, many with nuclei and also bacteria on the
surface of the cells.
All you have to do is explain the kid's blue finger tips to the parents.
If you want to explain cell division, we used to show them sections of
onion root tips stained with either Heidenhain's iron hematoxylin or
Feulgen. Barry
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
SMITH,REBEKAH FELICIA
Sent: Thursday, June 17, 2004 9:38 PM
To: CrochiereSteve <@t> aol.com; hodges420 <@t> msn.com;
histonet <@t> pathology.swmed.edu
Subject: Re: [Histonet] kids and histology
I don't know if this would be feasible in a job fair, but I
remember in grade school we did a science project involving
putting our cheek cells on slides and comparing them to onion
sections on slides. I thought it was fun at least. :-)
--
SMITH,REBEKAH FELICIA
"You are a child of the universe, no less than the trees and the
stars
You have a right to be here and whether or not it is clear to you,
no doubt the universe is unfolding as it should. Therefore be at
peace with G-d, whatever you conceive Him to be. And whatever your
labors and aspirations,in the noisy confusion of life, keep peace
in your soul.-Max Ehrmann,"Desiderata"
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 26
Date: Fri, 18 Jun 2004 14:52:54 +0100
From: "Marshall Terry Dr, Consultant Histopathologist"
<Terry.Marshall <@t> rothgen.nhs.uk>
Subject: RE: [Histonet] kids and histology
To: "Barry R Rittman" <Barry.R.Rittman <@t> uth.tmc.edu>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<FE2DB935F8BBB546B8A1BBF3459C5A1F02DC9E72 <@t> LIL.xRothGen.nhs.uk>
Content-Type: text/plain; charset="iso-8859-1"
Thanks Barry, but it was a rootorical question:-)
Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
Consultant Pathologist
Rotherham General Hospital
South Yorkshire
England
terry.marshall <@t> rothgen.nhs.uk
-----Original Message-----
From: Barry R Rittman [mailto:Barry.R.Rittman <@t> uth.tmc.edu]
Sent: 18 June 2004 14:44
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] kids and histology
Terry
It is trivia like this that my wife says that I worry about too much!!
Can use any root from any fast growing plant but onion is used because
it is continuing tradition and if you leave onions in the pantry too
long they will develop roots. Using a bulb, it is easy to place the bulb
suspended on the opening of a jar filled with water and harvest growing
roots as needed.
Perhaps the supply of onion root tips could be the start of a new
cottage industry?
Barry
-----Original Message-----
From: Marshall Terry Dr, Consultant Histopathologist
[mailto:Terry.Marshall <@t> rothgen.nhs.uk]
Sent: Friday, June 18, 2004 8:35 AM
To: Barry R Rittman; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] kids and histology
I've always wondered, "why onion?".
Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant
Pathologist Rotherham General Hospital South Yorkshire England
terry.marshall <@t> rothgen.nhs.uk
-----Original Message-----
From: Barry R Rittman [mailto:Barry.R.Rittman <@t> uth.tmc.edu]
Sent: 18 June 2004 14:29
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] kids and histology
Rebekah
I would also recommend these, at least as a first step.
It has the advantage of being simple and rapid.
We used Q-tips to rub the inside of the cheek, smeared this on a slide.
Either fix with alcohol or just dry. Stain with dilute toluidine blue
for a minutes or so, rinse and dry. While it is preferable to use
immersion oil, a lot of details can be seen just using dried sections.
Kids can see their own cells, many with nuclei and also bacteria on the
surface of the cells.
All you have to do is explain the kid's blue finger tips to the parents.
If you want to explain cell division, we used to show them sections of
onion root tips stained with either Heidenhain's iron hematoxylin or
Feulgen. Barry
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
SMITH,REBEKAH FELICIA
Sent: Thursday, June 17, 2004 9:38 PM
To: CrochiereSteve <@t> aol.com; hodges420 <@t> msn.com;
histonet <@t> pathology.swmed.edu
Subject: Re: [Histonet] kids and histology
I don't know if this would be feasible in a job fair, but I
remember in grade school we did a science project involving
putting our cheek cells on slides and comparing them to onion
sections on slides. I thought it was fun at least. :-)
--
SMITH,REBEKAH FELICIA
"You are a child of the universe, no less than the trees and the
stars
You have a right to be here and whether or not it is clear to you,
no doubt the universe is unfolding as it should. Therefore be at
peace with G-d, whatever you conceive Him to be. And whatever your
labors and aspirations,in the noisy confusion of life, keep peace
in your soul.-Max Ehrmann,"Desiderata"
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End of Histonet Digest, Vol 7, Issue 22
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