[Histonet] Human or animal tissue for H&E control, a difference??

[Bill Sinai]

Peggy,
I have to agree with you that liver is not always the best, my mentors, both
said the only way to ensure the nuclei were being adequately differentiated
was to look for an artery (easily seen in most tissues).  When these nuclei
were outstanding against the muscle then all others would most likely be
well stained.  Also the artery can be used to indicate good eosin staining,
as in one small area we have all the eosin staining components, (collagen,
muscle and red cells).

Bill Sinai
Laboratory Manager
Tissue Pathology, ICPMR
Westmead NSW 2145
Australia
Ph 02 9845 7774


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
lpwenk <@t> sbcglobal.net
Sent: Tuesday, 15 June 2004 7:36 PM
To: histonet <@t> lists.utsouthwestern.edu; Andrea Grantham
Subject: Re: [Histonet] Human or animal tissue for H&E control, a
difference??


I would like to politely disagree with liver as a control for nuclei in an
H&E. Since the nuclei are so large in the hepatocytes, and are always cut
open, the hematoxylin has to be REALLY bad before there is poor nuclear
staining. (in other words, most of the time, regardless of the quality of
the hematoxylin, the hepatocyte nuclei will show chromatin pattern.)
Granted, if one looks in the portal triad, there is a variety of nuclei
(connective tissue, muscle around blood vessels, cuboidal cells around bile
duct), but the vast majority of cells are large hepatocytes with large
nuclei with good chromatin pattern. And the hepatocytes seem to be what is
viewed, rather than the portal triad area, when doing a quick glance with
the microscope.

I agree with intestine and kidney, and lean towards small intestine myself.
Not only are the a wide variety of nuclei shapes, sizes and chromatin
pattern (lymphocytes, epithelial cells, muscle, etc.), but here is good
distribution of material to get the 3 shades of eosin seen (muscle in
several layers and blood vessels, connective tissue, and RBCs in blood
vessels). There are also goblet cells, which, if the hematoxylin is
approaching pH 3.0 or higher, really pick up the blue, so make  another good
indication of a problem with hematoxylin.

Of course, a multi-tissue block with intestine, kidney, and whatever your
most common tissues, or most troublesome tissues, would also work.

Just my 2 cents.

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073

----- Original Message -----
From: "Andrea Grantham" <algranth <@t> u.arizona.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, June 14, 2004 11:35 AM
Subject: RE: [Histonet] Human or animal tissue for H&E control, a
difference??


> I agree with Joe on the use of control tissue and might suggest using
> intestine, liver or maybe kidney as controls for H&E. You want to see nice
> crisp nuclear detail and I'd use something that shows the nuclei, probably
> not skin though.
> Andi Grantham
>
>
>
> At 10:03 AM 6/14/2004 -0500, you wrote:
> >Ian:
> >
> >I would focus on using control tissue from the species that your research
> >utilizes.  If you are working with human tissue, then go with a human
> >control.  If mouse, use mouse control, etc.   As to the use of skin for
> >nuclear detail, I would be skeptical.  I will let others make a
recommendation.
> >
> >Good luck,
> >
> >Joe Galbraith
> >
> >-----Original Message-----
> >From: histonet-bounces <@t> lists.utsouthwestern.edu
> >[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Bernard
> >Ian R SSgt 59 CRES/MSROP
> >Sent: Monday, June 14, 2004 9:10 AM
> >To: Histonet (E-mail)
> >Subject: [Histonet] Human or animal tissue for H&E control, a
> >difference??
> >Importance: High
> >
> >
> >Would it make a difference as to what tissue (animal or human) I use as
my
> >H&E control?  We are not CAP regulated, as we are in research.  Hence we
are
> >not required to run controls but simply as good management practice and
for
> >QC.
> >
> >Is skin the best tissue control as to show nuclear detail?
> >
> >
> >Thanks,
> >
> >SSgt B
> >
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> >Histonet <@t> lists.utsouthwestern.edu
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> >
> >_______________________________________________
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>
> ......................................................................
> : Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
> : Sr. Research Specialist       University of Arizona               :
> : (office:  AHSC 4212)          P.O. Box 245044                     :
> : (voice:  520-626-4415)        Tucson, AZ  85724-5044    USA       :
> : (FAX:  520-626-2097)          (email:  algranth <@t> u.arizona.edu)       :
> :...................................................................:
>            http://www.cba.arizona.edu/histology-lab.html
>
>
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