[Histonet] better perfusion and inclusion---thanks to y'all

[Nancy.Walker <@t> sanofi-synthelabo.com]

More precisions:
The catheter diameter varies with animal size. For a 35 g mouse a n° 4
cathater works well (inside diameter = 0.76 mm outside = 1.22 mm).

We used 5% sucrose (saccharose was a little french slipping in) in water ,
as you had suggested. Unfortunately I didn't check out if this was in fact
isotonic,  so that is a mistake! We perfused 1 minute at 300mm Hg. The
histology looked bad, that is,  the ventricles were dilated.

Another source advised us to use Mannitol at 25% for 20 " at 110 mm Hg.
This too created ventricle dilation.

For each trial 3 mice brains were processed, coronally sliced at the
hippocampal level and stained with H/E.

yours truely,
Nancy




----------------------------------------------------------
Le présent message, ainsi que les pièces ou annexes qui s'y trouvent
éventuellement jointes, s'adressent exclusivement à celles des personnes
qu'ils désignent comme destinataires. Constituant de ce fait une
correspondance privée à caractère confidentiel, leur contenu est protégé
par le secret des correspondances émises, transmises ou reçues par la voie
des télécommunications. Si ce message électronique vous est parvenu
fortuitement, veuillez avoir l'obligeance de le détruire, puis d'en aviser
l'expéditeur dans les meilleurs délais.

The Information in this e-mail belongs to Sanofi-Synthelabo, is intended
for the use of the individual or entity to which it is addressed, and may
contain information that is privileged, confidential, or exempt from
disclosure under applicable law. If you are not the intended recipient, you
are hereby notified that any disclosure, copying, distribution, or use of,
or reliance on, the contents of this e-mail is prohibited.  If you have
received this e-mail in error, please notify us immediately by replying
back to the sending e-mail address, and delete this e-mail message from
your computer.
----------------------------------------------------------