[Histonet] Toe nails !!!!!

[Thom Jensen]

   Hi Linda,

   I  have  cut  and  stained several nails including horse hoof.  Before
   processing  place  the  nail  in   10%  amonia water until bendable or
   rubbery  (approx.  2  hr to overnight depending on thickness).  Fix in
   formalin  and  process  as  usual.   For cutting, if the nail is still
   hard,  trim in and soak in amonia water again for 5 to 10 minutes.  To
   stick the sections to a slide I use Plus Charged Slides and 5 % Elmers
   Glue  in  a  coplin.   Dip  the  slides  in  the  glue and pick up the
   sections.   Allow  to  dry,  deparafinize and stain.  Amonia takes the
   keratin out of the nail and leaves it rubbery.

   good luck,

   Thom Jensen
   >From: "Linda  Jones" <ljones <@t> pathology.umsmed.edu>
   >To: <histonet <@t> lists.utsouthwestern.edu>
   >Subject: [Histonet] Toe nails !!!!!
   >Date: Wed, 09 Jun 2004 10:45:22 -0500
   >
   >Hi,
   >Do  anyone  know  how  to section and stain toe nails?  The nails are
   hard
   >to cut and the nail probably won't stay on the slide.
   >                                     Thanks
   >
   >Linda Harper-Jones,BS.HT. HTL(ASCP)
   >University of Mississippi Medical Center
   >Department of Pathology
   >Chief Histotechnologist Supervisor
   >(601) 984-1576
   >
   >Linda Harper-Jones,BS.HT. HTL(ASCP)
   >University of Mississippi Medical Center
   >Department of Pathology
   >Chief Histotechnologist Supervisor
   >(601) 984-1576
   >
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