[Histonet] IHC Staining Basics

[Julie Lee]

Hello from out here on the USA prairie!

We are a small hospital ( 1 full-time tech and 1 part-time tech). Our
pathologist would like us to start performing some more commonly requested
IHC stains (S-100, Vimentin, Cytokeratin, LCA). We are currently sending all
IHC to reference labs. This is a new undertaking for us. We are looking for
any suggestions as far as equipment (microwave, steamer ect.) and
methodologies for deparaffiniazation and antigen retrieval. The staining
will be done by hand. Any ideas for our situation would be greatly
appreciated!

Thanks, 
JulieAnne