[Histonet] Double immunofluorescence with Alexa Fluor

[Pablo Sánchez Quinteiro]

Thanks for your kind answer Dave. I'll go for 633, but I am still worry.
Would not the donkey anti-goat IgG conjugate to the goat anti-rabbit IgG?
In my protocol both secondary antibodies are incubated together.

I am very grateful for any input.

Pablo