[Histonet] Lymphocytes antibodies - Fixed tissue

[Patsy Ruegg]

Gabriel,
I use mouse monoclonal cd3 from Lab Vision for pan T cells on mouse tissue
ffpe, it should work great on frozen sections, unfortunately the t subsets
are not developed yet and work only on frozens so far.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Marceau,
Gabriel (IAF)(LAVAL)
Sent: Tuesday, June 08, 2004 8:53 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Lymphocytes antibodies - Fixed tissue


I Hi everyone,

I am looking for antibodies against lymphocytes (CD3 or other) that will
work on fixed tissue. Most of those comercially available are good for
cytometry but none is marked as usable on paraformaldehyde fixed tissue. If
someone tried one that is not specified for fixed tissue and it worked
please let me know. If not is there a product out there that is said to be
working in these conditions?

Gabriel
INRS-Institut Armand-Frappier

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