[Histonet] Re: Microscopic Bubbles...by the hundred

[Greg Dobbin]

From:           	Greg Dobbin <dobbin <@t> ACAD1.CS.UPEI.CA>
To:             	histonet-bounces <@t> lists.utsouthwestern.edu
Subject:        	(Fwd) Re: Microscopic Bubbles...by the hundred
Date sent:      	Tue, 8 Jun 2004 09:22:12 ADT


From:           	Greg Dobbin <dobbin <@t> ACAD1.CS.UPEI.CA>
To:             	Bruce Abaloz <brucea <@t> unimelb.edu.au>
Subject:        	Re: [Histonet] Microscopic Bubbles........by the hundreds & thousands
Date sent:      Tue, 8 Jun 2004 09:20:47 ADT

Hi Bruce,
This this is just a wild guess, but it sounds like you're up for any 
suggestions....
Maybe you need to look to the leaf structure to find the answer to 
the bubbles. Maybe the stomas (stomi?) are the source of the 
bubbles?? Are you cutting cross-sections of the leaves or planar 
(broad surface)? I would think the former would be less apt to have 
bubbles if indeed the stomas are the source.
Greg


Date sent:      	Tue, 08 Jun 2004 11:49:03 +1000
From:           	Bruce Abaloz <brucea <@t> unimelb.edu.au>
To:             	histonet-bounces <@t> lists.utsouthwestern.edu,
	histonet <@t> lists.utsouthwestern.edu
Subject:        	[Histonet] Microscopic Bubbles........by the hundreds & thousands

> Dear Histonetters,
> 
> We have  done the Oil Red O Charukian Method and Oil Red O technique 
> from Bancroft & Stevens, 3rd edition - both worked beautifully. Also 
> did Toluidine Blue stain combo with the Oil Red O & by itself - all 
> stunning results. Did the stains on cryo  -sections of a native 
> Australian plant to demonstrate that the 'grainy' bits on the 
> underside of the leaves were full of Lipids/fats.
>   The PROBLEM we have encountered, is -  no matter what Aqueous 
> Mountant we try , our cryosections are covered in microscopic air 
> bubbles (have tried to date: BDH/ Aquamount; DAKO /Faramount & 
> Glycergel & Ultramount.....even plain ol' Glycerol, (coverslipping 
> from DH20/Deionized H20/Tap H2O/Hot H2O/air dried - no H20)....... 
> ALL resulted with same problem.
> Can someone PLEASE advise what we are doing wrong (???) OR suggest a 
> possible answer as we need these results to be photographed ASAP. 
> Cheers & THANKS in advance,
> 
> Bruce in Melbourne/OZ
> -- 
> BRUCE ABALOZ                           PH:61383446282
> HISTOLOGIST                            FAX:61383447909
> DEPT.of ZOOLOGY                        EMAIL: brucea <@t> unimelb.edu.au
> THE UNIVERSITY Of MELBOURNE.
> VICTORIA.AUSTRALIA 3010          
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~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Greg Dobbin
Pathology Lab
Atlantic Veterinary College, U.P.E.I.
550 University Ave.
Charlottetown, P.E.I.
Canada,  C1A 4P3
Phone: (902)566-0744
Fax: (902)566-0851
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Making a living is getting; making a life is giving.