[Histonet] Was rat skin - now DeCloaker

[Ronald P. Wilson]

Thanks to all who responded to my problem with the rat skin disrupting. We
will try some of the tips with our next batch of staining. Someone had
recommended using Biocare's DeCloaker and Reveal solution. This raises the
next question - is there a definite advantage of using the DeCloaker over
the rice steamer. Certainly there is a big cost difference, $1200 vs. $25.
Is it worth the extra money? We have a lot of IHC work with a variety of
antibodies; Ki67, cytokeratins, cyclins, etc. ahead of us. I do have some
money to spend, so if the collective wisdom of Histoneters suggest that it
is worth the cost I will probably purchase one.

Ronald P. Wilson
Department of Comparative Medicine, HO54
Penn State University College of Medicine
M. S. Hershey Medical Center
500 University Drive
Hershey, PA 17033
phone:  717-531-8460
fax:       717-531-5001
e-mail:  rpw4 <@t> psu.edu