[Histonet] fibrin stain-Reply

[bhewlett <@t> cogeco.ca]

Fred,Peggy and Bryan,

The so-called WHO stain being referred to, is from one of the information sheets Bryan Llewellyn mentions.

It was a technique recommended for staining mixed tumours of the lung. Basically, it consisted of Alcian blue pH2.5
followed by the Masson HPS stain. Resulting in blue mucin and red keratin on a largely yellow background. Some fibrin
also stains red as a side benefit.
Early in the sixty's Atwood published a staining technique for the demonstration of inhaled amniotic fluid.
It consisted of Alcian Green at pH2.5(Alcian blue works just as well) followed by Lendrum's Phloxine-Tartrazine(HPT).
This also results in blue mucin and red keratin on a yellow background with some fibrin staining red as a side benefit.
Having tried both techniques, I much prefer Atwood's.
The yellowsolve component is more robust and easier to control.

Regards,

Bryan H.







>
> The WHO is not actually a method.  WHO stands for World Health
> Organisation and in the 1970's, or thereabouts, they published a series
> of information sheets recommending various histology lab procedures.
> The stain is likely one of those, and it may well have been an MSB,
> although I don't know for sure.  I do remember using their procedure for
> barium injection of hearts to get pictures of the blood vessels, though.
>
> If you want to demonstrate fibrin, keratin and mucin I would have
> thought Movat's pentachrome or one of its modifications would have been
> the most appropriate choice.
>
> Bryan Llewellyn
>
>
>
> lpwenk <@t> sbcglobal.net wrote:
> > What color is the mucin? If it's baby blue, the stain might be similar to a
> > Movat.
> >
> > Otherwise, my guess for a fibrin stain would be MSB. It's like a Masson
> > trichrome (with fibrin red, collagen blue, muscle red, RBC red, and I
> > believe mucin bluish) with Martius yellow dye added to make the RBCs yellow,
> > instead of the usual red with most trichromes. That makes it easier to find
> > fibrin when it is in clot areas.
> >
> > The original MSB used a double nuclear stain - Mayer hematoxylin followed by
> > celestin blue. And had somewhat unusual dyes - red being brilliant crystal
> > scarlet.
> >
> > We've modified it at our hospital. We use Weigert hematoxylin only, instead
> > of double blue dyes. Nuclei show up better. Then we use our regular blue and
> > red dyes of our Masson trichrome. Along with the Bouin post-mordant. The
> > only difference is the additional step of Martius yellow.
> >
> > Let me know if you need the MSB procedure.
> >
> > Otherwise, I'm waiting on the edge of my seat, to find out what a WHO stain
> > is. (And, yes, I did get the Townsend comment.)
> >
> > Peggy A. Wenk, HTL(ASCP)SLS
> > William Beaumont Hospital
> > Royal Oak, MI 48073
> >
> > ----- Original Message -----
> > From: "Fred Underwood" <funderwood <@t> mcohio.org>
> > To: <histonet <@t> lists.utsouthwestern.edu>
> > Sent: Friday, June 04, 2004 2:28 PM
> > Subject: [Histonet] fibrin stain
> >
> >
> >
> >>TGIF gang.
> >>
> >>I have a Doc trying to rule out an amniotic fluid embolism.  He is
> >>looking to demonstrate one or all of the following.  Fibrin, keratin,
> >>mucin.  One of the methods he mentioned was something called "the WHO"
> >>method, which utilizes celestine blue.  Of course very little procedural
> >>information was listed in the article.  I assume that Pete Townsend had
> >>nothing to do with it.  Any information you can offer would be greatly
> >>appreciated.  Thanks in advance.
> >>
> >>Fred Underwood
> >>Montgomery County Coroner's Office
> >>Dayton, OH
> >>
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