[Histonet] Nuclear staining input

Gary Gill garygill <@t> dcla.com
Thu Jun 3 16:29:42 CDT 2004


Hematoxylin solution is on glide path to overoxidation.

Gary Gill

-----Original Message-----
From: Bernard Ian R SSgt 59 CRES/MSROP [mailto:Ian.Bernard <@t> LACKLAND.AF.MIL] 
Sent: Thursday, June 03, 2004 4:22 PM
To: Histonet (E-mail)
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Nuclear staining input


Staining H&E problem.  Hematoylin is greyish more than purple. Use Ammmonia
water and acid alcohol in steps.

What are potential problems.
Never had issue before.

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