[Histonet] RE: Info required on antibodies

[C.M. van der Loos]

Dear Carmen,
We successfully used the following on FFPE sections (HIER: Tris/EDTA pH9.0 for 15 min at 100C in microwave and 10 min cool-down):
Kip1/p27 from Dako (M 7203) using an 1:100 dilution (60 min, RT) and detection with EnVision+ GAM/HRP. DAB+ as chromogen. 
Fli-1 from Santa Cruz (# sc-356) using a 1:2000 dilution (overnight 4C) and detection with PowerVision-GAR/AP (www.immunovisiontech.com). Permanent Red (Dako) as chromogen.
Hope this helps!

Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center
Amsterdam - The Netherlands

----- Original Message ----- 
>From  carmen loiselle <carmen_loiselle <@t> hotmail.com> 
Date  Tue, 01 Jun 2004 11:46:37 -0400 
To  histonet <@t> pathology.swmed.edu 
Subject  [Histonet] Info required on antibodies 
Hello everyone,

Does anyone have any information regarding the following antibodies;  
P19(INK4d), Kip1/P27 and fli-1 ???  These antibodies would be used on 
paraffin sections for immunohistochemistry .  We  also have  Ventana 
immunostainer (NEXES and Benchmark) .


Any input would be greatly appreciated . Thank you in advance.

Carmen