[Histonet] TRAP staining

[Jackie.O'Connor <@t> abbott.com]

As Patsy mentioned, I've had continuing successful TRAP staining on 
formalin fixed (24-48 hours), Formic acid decalcified mouse legs (6 hours 
decal) - and since I am on a learning curve - the same protocol does NOT 
work with HcL decalcification - although I must admit, I don't know why.

Jackie O'Connor
Abbott Laboratories
Discovery Chemotherapeutics




"Patsy Ruegg" <pruegg <@t> colobio.com>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
06/01/2004 11:51 AM

 
        To:     <Myri37 <@t> aol.com>, <histonet <@t> pathology.swmed.edu>
        cc: 
        Subject:        RE: [Histonet] TRAP staining


How are your fresh cells fixed?  In my experience aldehyde fixed paraffin
processed tissue especially decalcified is not the best for demonstrating
osteoclasts with TRAP, although Jackie O'Connor at Abbott has gotten it to
work on formalin fixed tissue if the fixation was not very long.  I use 
TRAP
on cold methanol fixed bone biopsie's processed without decalcification 
into
glycol methacrylate.  I would think that the fresh fixed cells should work
fine.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
Myri37 <@t> aol.com
Sent: Tuesday, June 01, 2004 8:11 AM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] TRAP staining


Hi
I would like to try SIGMA kit N°85, for staining osteoclats on fresh fixed
cells (reconstructed tissue, and also paraffin embedded secions. Tissue is
fixed in 4 % paraformaldehyde, is there another fixative wich works better 
?
Do you think if i must change the way to proceede ?

Thank you very much
Myriam baali
Natural implant


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