Fwd: Re: [Histonet] Rodent Processing Schedule?

[Atoska S. Gentry]

>Date: Fri, 30 Jul 2004 16:20:34 -0500
>To: "Timothy R. Wheelock" <twheelock <@t> mclean.harvard.edu>
>From: "Atoska S. Gentry" <gentras <@t> vetmed.auburn.edu>
>Subject: Re: [Histonet] Rodent Processing Schedule?
>
>
>
>Hello, please will you share your replies with me. I was about to make a 
>similar inquiry, except mine is from hindsight. I processed a couple of 
>whole rat brains on my four hour serial section rodent tissue process 
>cycle and unfortunately I'm experiencing hollow spaces in areas of the 
>brain upon sectioning. I'm wondering if this is a result of either 
>inadequate fixation or processing. Thanks, Atoska
>
>
>At 08:48 AM 7/30/04, you wrote:
>>Hi Everyone:
>>
>>Does anyone have a protocol for  processing whole, formalin fixed mouse 
>>brains? The mouse brains need to be processed whole.
>>I am concerned that  over-processing, using a schedule meant for human 
>>brain tissue, will dry the tissue out excessively and make the serial 
>>sectioning a nightmare.
>>Also, would the same schedule apply to whole rat brains, or should the 
>>times be increased a bit?
>>Thank you.
>>
>>Tim Wheelock
>>Harvard Brain Tissue Resource Center
>>McLean Hospital
>>Belmont MA
>>
>>
>>
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>
>Atoska S. Gentry B.S., HT(ASCP)
>Research Assistant III
>Scott-Ritchey Research Center
>College of Veterinary Medicine
>Auburn University, AL  36849
>Phone# (334)844-5579  Fax# (334)844-5850

Atoska S. Gentry B.S., HT(ASCP)
Research Assistant III
Scott-Ritchey Research Center
College of Veterinary Medicine
Auburn University, AL  36849
Phone# (334)844-5579  Fax# (334)844-5850