[Histonet] theoretical question on fixing and section (formalin and cryosections)

ZP degaboh <@t> rice.edu
Fri Jul 30 12:22:39 CDT 2004


Hello all, 

This question refers to bone tissue.  If you want to do IHC, cryosections
generally give better results, right?  Does it make sense to fix the tissue
in 10% formalin, decalcify (say in formic acid), and then "embed" in OCT and
freeze in dry ice to take cryosections?   It just seems like people do 10%
formalin and follow that with paraffin embedding..

Thanks!

Zarana Patel
degaboh <@t> rice.edu





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