[Histonet] silanized slides

Dorothy.L.Webb <@t> HealthPartners.Com Dorothy.L.Webb <@t> HealthPartners.Com
Wed Jul 28 10:20:33 CDT 2004


Fill slide racks with glass slides and soak for 2 minutes in: 490 ml. of
Absolute Alcohol containing 10 ml. of 3-aminopropyltriethoxysilane
(Sigma#A3648, 100 ml@$15.75)

Rinse well in 3 changes of distilled water.  Change distilled water after 10
racks.

Drain and place slides in oven at 60 degrees C for 30 minutes, or until dry.

Store slides in dust free box, as everything will stick to them (we use the
boxes the slides come in)

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