[Histonet] APES/positively charged slides

Amy Porter portera203 <@t> yahoo.com
Wed Jul 28 09:16:32 CDT 2004


Christie - we dry all of our plastic sections overnight in a 60 C. oven - we have found that this helps retain the sections on the slide through most pretreatments that we use.  Hope this idea helps you out.  

Christie M Quinlan <cquinlan <@t> wlgore.com> wrote:Hi all,
I am having problems with plastic sections (MMA) coming off of APES-subbed
slides during deplasticization. I don't want to use gelatin for these as
they are for immuno. Has anyone else encountered this problem? If so,
what method worked for you? Is there any interference with APES solution
and the positive charge on PLUS slides? Thanks in advance for any
suggestions.


Christie Quinlan
W.L. Gore and Assoc.
Flagstaff, AZ
(928)864-3938



_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



Amy S.Porter, HT(ASCP) 
Michigan State University 
Department of Physiology 
Division of Human Pathology 
College of Human Medicine 
portera203 <@t> yahoo.com


		
---------------------------------
Do you Yahoo!?
Yahoo! Mail Address AutoComplete - You start. We finish.



More information about the Histonet mailing list