[Histonet] controls for immunos and special stains
Galbraith, Joe
joseph-galbraith <@t> uiowa.edu
Tue Jul 27 15:26:01 CDT 2004
Julie:
You are required by CAP to run negative controls for immunos that are specific for the species of the primary antibody AND for EACH pretreatment utilized. There are even some rumblings about isotype specific controls as well but no specific requirement for that as yet to my knowledge. Naturally you must have a positive control as well. Our positive controls are on the same slide as the patient tissue section. We run positive controls that contain elements that stain and don't stain with the primary antibody. The negative controls are patient tissue sections cut onto separate slides and stained (hopefully negative) with species specific immunoglobulins using each pretreatment method. In a worst case scenario there will be one negative control for each test slide run.
Ideally your special stain positive controls should contain elements that stain and elements that do not stain. These should also be 'same slide' controls when possible. The elements of your positive control that are known to NOT stain could act as a negative control. Of course the best negative control, if you can find one, would be the same tissue element (ie the same tumor type for example) where one piece was known to have a positive reaction and another piece was known to be negative.
We will often include a normal and abnormal tissue in the on-slide control as well. For example, a piece of normal lymph node and a piece of lymphoma lymph node for a Bcl-2 control. This approach provides a nice contrast when there are elements that stain in normal as well as abnormal tissue.
Best wishes,
Joe Galbraith
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
Julie.Sanders <@t> med.va.gov
Sent: Tuesday, July 27, 2004 12:31 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] controls for immunos and special stains
I am having a "discussion" with our lab chief concerning the use of controls
with special stain and immunos. He insists that I run a negative control
with special stains and a "known negative" control for immunos. Currently
we run only a positive control for specials and a postive control and
negative patient for immunos.
Thoughts and/or protocols would be more than welcome!
Thanks,
Julie
Julie Sanders
Supervisor, Anatomic Pathology
VAMC, Cincinnati, Ohio
-----Original Message-----
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Sent: Tuesday, July 27, 2004 1:05 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 8, Issue 39
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Today's Topics:
1. Securline markers (Emily)
2. RE: Securline markers (Bartlett, Jeanine)
3. re silane from sigma (Carl)
4. re microscope filters (Carl)
5. RE: Securline markers (Poteete, Jacquie A.)
6. re microscope filters (Carl)
7. RE: Securline markers (Joe Nocito)
8. Re: Securline markers (Jo Dee Fish)
9. RE: Securline markers (Connie McManus)
10. paraffin blocks (Ernestine Middleton)
11. Re: paraffin blocks (Bill Blank)
12. soften formalin fixed samples (Leclerc Jocelyne)
13. Re: P504S (Dana Settembre)
14. AE 13 stain? (Featherstone, Annette)
15. Re: Securline markers (Rebecca Barnhart)
16. RE: Securline markers (Rebecca Barnhart)
17. Re: AE 13 stain? (Andi Kappeler)
18. AE13 (Michael Fredrickson)
19. Amino Silane coated slides (Sharon.Davis-Devine)
20. RE: Securline markers (Connie McManus)
21. RE: paraffin blocks (Connie McManus)
22. Re: soften formalin fixed samples
(mari.ann.mailhiot <@t> leica-microsystems.com)
23. Re: soften formalin fixed samples (Gayle Callis)
24. RE: soften formalin fixed samples
(Marshall Terry Dr, Consultant Histopathologist)
25. stain for mast cells (Winters, Bert)
26. I have not received any histonet mail in a while
(Stanley.Lupo <@t> gsk.com)
27. Re: AE 13 stain? (Dana Settembre)
----------------------------------------------------------------------
Message: 1
Date: Mon, 26 Jul 2004 10:46:43 -0700 (PDT)
From: Emily <emmie222 <@t> yahoo.com>
Subject: [Histonet] Securline markers
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20040726174643.38022.qmail <@t> web41202.mail.yahoo.com>
Content-Type: text/plain; charset=us-ascii
We have been having a major problem the Securline markers for about a year -
they are pretty much dry when we get them. The company that manufactures
them told me to add xylene to each marker. It works most of the time, but it
takes up a lot of time to add the xylene. My feeling is that I shouldn't
have to do that anyway - they should just make a marker that actually works.
I have tried out many other pens/markers and have found none to be
satisfactory. The ink either fades too much during processing or just
totally washes off. Does anyone have any similar issues? Does anyone have
any ideas or suggestions?
------------------------------
Message: 2
Date: Mon, 26 Jul 2004 13:58:14 -0400
From: "Bartlett, Jeanine" <JQB7 <@t> CDC.GOV>
Subject: RE: [Histonet] Securline markers
To: "Emily" <emmie222 <@t> yahoo.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CB857F6460D42E4AAEA195054A25406C02F5EB1C <@t> m-ncid-2.NCID.CDC.GOV>
Content-Type: text/plain; charset="US-ASCII"
Always a problem. Try the Statmart pens from StatLab. Maybe Leslie
will send you a free sample! 1-800-442-3573 X225
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Emily
Sent: Monday, July 26, 2004 1:47 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Securline markers
We have been having a major problem the Securline markers for about a
year - they are pretty much dry when we get them. The company that
manufactures them told me to add xylene to each marker. It works most of
the time, but it takes up a lot of time to add the xylene. My feeling is
that I shouldn't have to do that anyway - they should just make a marker
that actually works. I have tried out many other pens/markers and have
found none to be satisfactory. The ink either fades too much during
processing or just totally washes off. Does anyone have any similar
issues? Does anyone have any ideas or suggestions?
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 3
Date: Mon, 26 Jul 2004 19:04:04 +0100
From: "Carl" <carl.hobbs <@t> kcl.ac.uk>
Subject: [Histonet] re silane from sigma
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000e01c4733a$f01baee0$31292bd9 <@t> home>
Content-Type: text/plain; charset="iso-8859-1"
Yes....used it for years. Works well, dependant on your protocol, of course
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Message: 4
Date: Mon, 26 Jul 2004 19:08:41 +0100
From: "Carl" <carl.hobbs <@t> kcl.ac.uk>
Subject: [Histonet] re microscope filters
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001901c4733b$95301ec0$31292bd9 <@t> home>
Content-Type: text/plain; charset="iso-8859-1"
You should be able to adjust white balance successfully with a digital
camera; there are also the "colour sliders" for fine balance , in any good
software.. However, when taking pics of DAB immuno with Hx counterstain I
always use an 80A daylight (blue) filter to balance out the yellowish cast
of light one gets from a std microscope bulb. Even for my checking
microscope, I'll use that type of filter.
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Message: 5
Date: Mon, 26 Jul 2004 13:07:28 -0500
From: "Poteete, Jacquie A." <japoteete <@t> saintfrancis.com>
Subject: RE: [Histonet] Securline markers
To: 'Emily' <emmie222 <@t> yahoo.com>, histonet <@t> lists.utsouthwestern.edu
Message-ID:
<D423BBB85E91D411A38D42003000996506973EA4 <@t> mailnt1.saintfrancis.com>
Content-Type: text/plain
I think we've been here before, but I wasn't able to pull it out of the
archives. Our lab switched to Statmark pens from Statlab (1-800-442-3573),
and we have had no problems at all.
Jacquie Poteete MT(ASCP)QIHC
Lead Technologist, IHC Laboratory
Saint Francis Hospital, Tulsa. OK
japoteete <@t> saintfrancis.com
> -----Original Message-----
> From: Emily [SMTP:emmie222 <@t> yahoo.com]
> Sent: Monday, July 26, 2004 12:47 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Securline markers
>
> We have been having a major problem the Securline markers for about a year
> - they are pretty much dry when we get them. The company that manufactures
> them told me to add xylene to each marker. It works most of the time, but
> it takes up a lot of time to add the xylene. My feeling is that I
> shouldn't have to do that anyway - they should just make a marker that
> actually works. I have tried out many other pens/markers and have found
> none to be satisfactory. The ink either fades too much during processing
> or just totally washes off. Does anyone have any similar issues? Does
> anyone have any ideas or suggestions?
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
********* Email Confidentiality Statement *********
Visit http://www.saintfrancis.com/emailconf.asp
------------------------------
Message: 6
Date: Mon, 26 Jul 2004 19:13:29 +0100
From: "Carl" <carl.hobbs <@t> kcl.ac.uk>
Subject: [Histonet] re microscope filters
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <002201c4733c$41097f70$31292bd9 <@t> home>
Content-Type: text/plain; charset="iso-8859-1"
Goodness me, Barry.....Didymium filters takes me back to those old days of
"fine-tuning" the image to get a quality image of a H&E. Thanks for
reminding me. AND the dilemma as to whether to use critical illumination to
get "the best" image, as those Photomicrographic books used to recommend, if
I recall correctly.
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------------------------------
Message: 7
Date: Mon, 26 Jul 2004 13:22:11 -0500
From: "Joe Nocito" <JNocito <@t> Pathreflab.com>
Subject: RE: [Histonet] Securline markers
To: "Emily" <emmie222 <@t> yahoo.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <JFEMICGBHEGPLAMIJPJPOEIFCFAA.JNocito <@t> Pathreflab.com>
Content-Type: text/plain; charset="us-ascii"
Emily,
I had several nightmares with those pens. Statlab Medical Products,
up by
Dallas, 1-800-442-3573 have a new pen that works better. The downfall of
these pens is that you have to let the ink dry completely before placing
them in solutions. Once the ink is thoroughly dry, we haven't had a problem.
The ink is still dark after processing and the pens last about twice as long
as the other brand. I think their web page is www.statlab.com
Good luck.
Joe Nocito, BS, HT(ASCP) QIHC
Histology Manager
Pathology Reference Lab
San Antonio, TX
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Emily
Sent: Monday, July 26, 2004 12:47 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Securline markers
We have been having a major problem the Securline markers for about a year -
they are pretty much dry when we get them. The company that manufactures
them told me to add xylene to each marker. It works most of the time, but it
takes up a lot of time to add the xylene. My feeling is that I shouldn't
have to do that anyway - they should just make a marker that actually works.
I have tried out many other pens/markers and have found none to be
satisfactory. The ink either fades too much during processing or just
totally washes off. Does anyone have any similar issues? Does anyone have
any ideas or suggestions?
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Mon, 26 Jul 2004 11:39:28 -0700
From: Jo Dee Fish <jfish <@t> gladstone.ucsf.edu>
Subject: Re: [Histonet] Securline markers
To: Emily <emmie222 <@t> yahoo.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <v04210102bd2b0018bc96@[10.40.2.123]>
Content-Type: text/plain; charset="us-ascii" ; format="flowed"
Emily,
We use RediSharp Plus from Dixon. I order mine from www.tedpella.com.
These pens are great and last a long, long time. Available in black and
blue.
Take care,
Jo Dee
**********************************************************************
**********
Jo Dee Fish
Research Technologist III
Gladstone Institute of Cardiovascular Disease
Telephone: (415) 695-3720
Fax: (415) 285-5632
E-mail: jfish <@t> gladstone.ucsf.edu
Mailing address:
Gladstone Institutes
P.O. Box 419100
San Francisco, CA 94141-9100
------------------------------
Message: 9
Date: Mon, 26 Jul 2004 15:50:03 -0600
From: "Connie McManus" <convmcm <@t> cc.usu.edu>
Subject: RE: [Histonet] Securline markers
To: "'Emily'" <emmie222 <@t> yahoo.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001d01c4735a$81a6a710$4a737b81 <@t> Cygnus>
Content-Type: text/plain; charset="us-ascii"
I stopped using secureline markers some time ago because the writing on
an entire rack of slides came off completely. I agree with you...you
shouldn't have to add xylene to pens. They should have better QC than
that. We use a pencil to write the accession number on the slides before
we cut, then apply paper sticky labels after cover slipping. The
information is set up in MS Excel and printed on large label paper. This
is time intensive, but we don't have a slide labeler and I can't think
of any other options. If there are any out there, I'm all ears, too *g*
Connie McManus
Utah Veterinary Diagnostics Laboratory
Utah State University
Logan, UT
Phone: 435/797-1891
fax: 435/797-2805
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Emily
Sent: Monday, July 26, 2004 10:47 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Securline markers
We have been having a major problem the Securline markers for about a
year - they are pretty much dry when we get them. The company that
manufactures them told me to add xylene to each marker. It works most of
the time, but it takes up a lot of time to add the xylene. My feeling is
that I shouldn't have to do that anyway - they should just make a marker
that actually works. I have tried out many other pens/markers and have
found none to be satisfactory. The ink either fades too much during
processing or just totally washes off. Does anyone have any similar
issues? Does anyone have any ideas or suggestions?
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 10
Date: Mon, 26 Jul 2004 21:02:40 -0400 (EDT)
From: Ernestine Middleton <ernestinemiddleton <@t> yahoo.ca>
Subject: [Histonet] paraffin blocks
To: histonet <@t> pathology.swmed.edu
Message-ID: <20040727010240.72170.qmail <@t> web51502.mail.yahoo.com>
Content-Type: text/plain; charset=us-ascii
Hi;
I like to know how do other Histology Lab. get rid of paraffin blocks that
are 20+years. Do you have a medical waste company destroy them or do you
do it your self?
Thank you for answer me as quickly as possible.
Ernestine Middleton, Manager
Montefiore Med. Ct.
Bronx, NY
914-920-4157
---------------------------------
Post your free ad now! Yahoo! Canada Personals
------------------------------
Message: 11
Date: Mon, 26 Jul 2004 20:51:18 -0500
From: Bill Blank <bill501 <@t> mindspring.com>
Subject: Re: [Histonet] paraffin blocks
To: Ernestine Middleton <ernestinemiddleton <@t> yahoo.ca>,
histonet <@t> pathology.swmed.edu
Message-ID: <p06110408bd2b65764df4@[63.153.12.58]>
Content-Type: text/plain; charset="us-ascii" ; format="flowed"
At 9:02 PM -0400 7/26/04, Ernestine Middleton wrote:
>Hi;
>I like to know how do other Histology Lab. get rid of paraffin
>blocks that are 20+years. Do you have a medical waste company
>destroy them or do you do it your self?
We have a medical waste company destroy them.
Bill
--
______________
Bill Blank, MD
Heartland Lab, Inc
------------------------------
Message: 12
Date: Tue, 27 Jul 2004 11:16:15 +0200
From: Leclerc Jocelyne <leclercj <@t> vetanat.unizh.ch>
Subject: [Histonet] soften formalin fixed samples
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BD2BE9FF.283E%leclercj <@t> vetanat.unizh.ch>
Content-Type: text/plain; charset="ISO-8859-1"
Good morning everybody!
I have a problem with my formalin fixed samples: they are just too hard. Now
I wonder if there is a possibility to soften or "remoisture" them.
I would be very pleased if anyone of you felt able to help me.
Thank you and best regards,
Jocelyne
--
VetSuisse Fakultät
Veterinär-Anatomisches Institut
Winterthurerstr. 260
8057 Zürich
------------------------------
Message: 13
Date: Tue, 27 Jul 2004 07:25:28 -0400
From: Dana Settembre <settembr <@t> umdnj.edu>
Subject: Re: [Histonet] P504S
To: Rcartun <@t> harthosp.org, histonet <@t> lists.utsouthwestern.edu
Message-ID: <s1060399.071 <@t> smtpnpc.umdnj.edu>
Content-Type: text/plain; charset=US-ASCII
Sorry, Using Biocare Medical's AMACR which is Research Use Only.
Dana Settembre
University Hospital - UMDNJ
Newark, NJ
>>> Richard Cartun <Rcartun <@t> harthosp.org> 7/23/2004 5:14:14 PM >>>
Is anyone using an antibody to "alpha-methylacyl-CoA racemase" (P504S)
that is labeled "IVD" or "ASR"? Thank you!
Richard Cartun
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 14
Date: Tue, 27 Jul 2004 07:28:34 -0400
From: "Featherstone, Annette" <AFeatherstone <@t> KaleidaHealth.Org>
Subject: [Histonet] AE 13 stain?
To: 'Emily' <emmie222 <@t> yahoo.com>, histonet <@t> lists.utsouthwestern.edu
Message-ID:
<DF6AB9298498D31199CF0008C7E6C1200CF95DD3 <@t> kalmb02.kaleidahealth.org>
Content-Type: text/plain; charset="iso-8859-1"
Does anyone know what AE-13 is? Is it an immunostain, and what is it for?
Annette Featherstone HT/MLT
-----Original Message-----
From: Emily [mailto:emmie222 <@t> yahoo.com]
Sent: Monday, July 26, 2004 13:47
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Securline markers
We have been having a major problem the Securline markers for about a year -
they are pretty much dry when we get them. The company that manufactures
them told me to add xylene to each marker. It works most of the time, but it
takes up a lot of time to add the xylene. My feeling is that I shouldn't
have to do that anyway - they should just make a marker that actually works.
I have tried out many other pens/markers and have found none to be
satisfactory. The ink either fades too much during processing or just
totally washes off. Does anyone have any similar issues? Does anyone have
any ideas or suggestions?
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Message: 15
Date: Tue, 27 Jul 2004 07:54:33 -0400
From: "Rebecca Barnhart" <RBARNHART <@t> summithealth.org>
Subject: Re: [Histonet] Securline markers
To: <histonet <@t> lists.utsouthwestern.edu>,<emmie222 <@t> yahoo.com>
Message-ID: <s1060a4b.055 <@t> ch-groupie.summithealth.local>
Content-Type: text/plain; charset=US-ASCII
We have tried many different pens for writing on cassettes and slides,
pencils smear for us. The best that we have found is Shur/Mark from
Triangle Biomedical Sciences. They have replaceable tips so when they
dry up you just put a new tip in. We keep one for writing on cassettes
and one for slides (writing on slides makes the pen dull and then hard
to write on a cassette). I just received a sample from Pacific
Southwest Lab Equipment that so far has done well, PathPen. So far we
have used it to write on cassettes and slides and both stayed on. The
only thing you MUST let the ink dry, they say for 60 seconds, or it will
smear.
Becky
>>> Emily <emmie222 <@t> yahoo.com> 07/26/04 01:46PM >>>
We have been having a major problem the Securline markers for about a
year - they are pretty much dry when we get them. The company that
manufactures them told me to add xylene to each marker. It works most of
the time, but it takes up a lot of time to add the xylene. My feeling is
that I shouldn't have to do that anyway - they should just make a marker
that actually works. I have tried out many other pens/markers and have
found none to be satisfactory. The ink either fades too much during
processing or just totally washes off. Does anyone have any similar
issues? Does anyone have any ideas or suggestions?
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 16
Date: Tue, 27 Jul 2004 08:01:30 -0400
From: "Rebecca Barnhart" <RBARNHART <@t> summithealth.org>
Subject: RE: [Histonet] Securline markers
To: <convmcm <@t> cc.usu.edu>,<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s1060bfc.063 <@t> ch-groupie.summithealth.local>
Content-Type: text/plain; charset=US-ASCII
We get preprinted xylene resistant labels to use for our paps only. Our
liquid base are sent elsewhere for processing and that is what they use,
so we started to just for our paps. We get the labels from Surgipath.
We told them how we wanted the labels printed (our numbering system,
label and ink color, size and our hospital name on them) For our
surgical and non-gyn our computer system prints regular labels. Do you
have a computer system that you accession the cases in, like Meditech,
Sunquest, CoPath etc.?
Becky
>>> "Connie McManus" <convmcm <@t> cc.usu.edu> 07/26/04 05:50PM >>>
I stopped using secureline markers some time ago because the writing
on
an entire rack of slides came off completely. I agree with you...you
shouldn't have to add xylene to pens. They should have better QC than
that. We use a pencil to write the accession number on the slides
before
we cut, then apply paper sticky labels after cover slipping. The
information is set up in MS Excel and printed on large label paper.
This
is time intensive, but we don't have a slide labeler and I can't think
of any other options. If there are any out there, I'm all ears, too
*g*
Connie McManus
Utah Veterinary Diagnostics Laboratory
Utah State University
Logan, UT
Phone: 435/797-1891
fax: 435/797-2805
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Emily
Sent: Monday, July 26, 2004 10:47 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Securline markers
We have been having a major problem the Securline markers for about a
year - they are pretty much dry when we get them. The company that
manufactures them told me to add xylene to each marker. It works most
of
the time, but it takes up a lot of time to add the xylene. My feeling
is
that I shouldn't have to do that anyway - they should just make a
marker
that actually works. I have tried out many other pens/markers and have
found none to be satisfactory. The ink either fades too much during
processing or just totally washes off. Does anyone have any similar
issues? Does anyone have any ideas or suggestions?
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 17
Date: Tue, 27 Jul 2004 14:08:42 +0200
From: "Andi Kappeler" <kappeler <@t> patho.unibe.ch>
Subject: Re: [Histonet] AE 13 stain?
To: "Histonet" <HistoNet <@t> pathology.swmed.edu>
Message-ID: <009c01c473d2$75c88b20$27955c82 <@t> patho.unibe.ch>
Content-Type: text/plain; charset="iso-8859-1"
What is probably meant here is a cytokeratin marker which most often is sold
as a cocktail of 2 monoclonal antibodies, namely AE1 and AE3.
AE1 reacts with an epitope present on cytokeratins 10, 13, 14, 15, 16, 19,
AE3 binds to an epitope found on cytokeratins 1, 2, 3, 4, 5, 6, 7, 8. The
cocktail is often used as a 'pan-cytokeratin marker' like clone MNF116 or
clone Lu-5.
Hope this helps
Andi Kappeler
Institute of Pathology, University of Bern, Switzerland
----- Original Message -----
From: "Featherstone, Annette" <AFeatherstone <@t> KaleidaHealth.Org>
To: "'Emily'" <emmie222 <@t> yahoo.com>; <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, July 27, 2004 1:28 PM
Subject: [Histonet] AE 13 stain?
> Does anyone know what AE-13 is? Is it an immunostain, and what is it for?
> Annette Featherstone HT/MLT
>
------------------------------
Message: 18
Date: Tue, 27 Jul 2004 08:42:02 -0400
From: "Michael Fredrickson" <mfredrickson <@t> cohenderm.com>
Subject: [Histonet] AE13
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <3D55809F621E7C438DA80098CDE3744D0545CA <@t> hub.cohenderm.com>
Content-Type: text/plain; charset="iso-8859-1"
AE 13 is an antibody specific for pilar keratin. Please reference: Arch
Dermatol 1990 Feb;126(2):189-94
Mike Fredrickson, Cohen Dermatopathology
------------------------------
Message: 19
Date: Tue, 27 Jul 2004 07:47:09 -0500
From: "Sharon.Davis-Devine" <Sharon.Davis-Devine <@t> carle.com>
Subject: [Histonet] Amino Silane coated slides
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E0DA23E35D80D611B80600A0C9EA33D5025E66C7 <@t> exchange1.carle.com>
Content-Type: text/plain
I am looking for anyone who would be willing to share their protocol for
coating of Amino Silane slides. Thanks in advance.
Sharon Davis-Devine, CT (ASCP)
Technical Specialist of Cytology
Carle Clinic
602 West University Ave.
Urbana, Illinois 61801
Phone: 217-383-3402
sharon.davis-devine <@t> carle.com
------------------------------
Message: 20
Date: Tue, 27 Jul 2004 08:34:30 -0600
From: "Connie McManus" <convmcm <@t> cc.usu.edu>
Subject: RE: [Histonet] Securline markers
To: "'Rebecca Barnhart'" <RBARNHART <@t> summithealth.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000001c473e6$d40c3a60$4a737b81 <@t> Cygnus>
Content-Type: text/plain; charset="us-ascii"
Becky,
We don't have any of the software systems you clinical folks use. I
just make my labels in MicroSoft Excel -- I took the time to create a
template where the cell size is the same dimensions as the frosted end
of our slides (this took some trial and error). I formatted one cell
with the font size and other attributes we wanted, then copy - pasted a
bunch of cells with this formatting. After we embed our blocks, one of
us goes to the file, types in the accession numbers, the day's date and
then print it on large label paper. It works really well for us except
that it IS time consuming. We have found that the Surgipath labels you
use don't work well for us. We tried other labels - Nalgene Nunc have
some poly paper laser labels that are supposed to be set up in MS Word,
but that didn't work for us very well, either. Sometimes the shortest,
best, most efficient way is the way you know how to do.
Connie McManus
Utah Veterinary Diagnostics Laboratory
Utah State University
Logan, UT
Phone: 435/797-1891
fax: 435/797-2805
where am i going and why am i in this handbasket???
-----Original Message-----
From: Rebecca Barnhart [mailto:RBARNHART <@t> summithealth.org]
Sent: Tuesday, July 27, 2004 5:02 AM
To: convmcm <@t> cc.usu.edu; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Securline markers
We get preprinted xylene resistant labels to use for our paps only. Our
liquid base are sent elsewhere for processing and that is what they use,
so we started to just for our paps. We get the labels from Surgipath.
We told them how we wanted the labels printed (our numbering system,
label and ink color, size and our hospital name on them) For our
surgical and non-gyn our computer system prints regular labels. Do you
have a computer system that you accession the cases in, like Meditech,
Sunquest, CoPath etc.?
Becky
>>> "Connie McManus" <convmcm <@t> cc.usu.edu> 07/26/04 05:50PM >>>
I stopped using secureline markers some time ago because the writing
on
an entire rack of slides came off completely. I agree with you...you
shouldn't have to add xylene to pens. They should have better QC than
that. We use a pencil to write the accession number on the slides
before
we cut, then apply paper sticky labels after cover slipping. The
information is set up in MS Excel and printed on large label paper.
This
is time intensive, but we don't have a slide labeler and I can't think
of any other options. If there are any out there, I'm all ears, too
*g*
Connie McManus
Utah Veterinary Diagnostics Laboratory
Utah State University
Logan, UT
Phone: 435/797-1891
fax: 435/797-2805
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Emily
Sent: Monday, July 26, 2004 10:47 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Securline markers
We have been having a major problem the Securline markers for about a
year - they are pretty much dry when we get them. The company that
manufactures them told me to add xylene to each marker. It works most
of
the time, but it takes up a lot of time to add the xylene. My feeling
is
that I shouldn't have to do that anyway - they should just make a
marker
that actually works. I have tried out many other pens/markers and have
found none to be satisfactory. The ink either fades too much during
processing or just totally washes off. Does anyone have any similar
issues? Does anyone have any ideas or suggestions?
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 21
Date: Tue, 27 Jul 2004 08:37:15 -0600
From: "Connie McManus" <convmcm <@t> cc.usu.edu>
Subject: RE: [Histonet] paraffin blocks
To: "'Ernestine Middleton'" <ernestinemiddleton <@t> yahoo.ca>,
<histonet <@t> pathology.swmed.edu>
Message-ID: <000101c473e7$35f9f5f0$4a737b81 <@t> Cygnus>
Content-Type: text/plain; charset="us-ascii"
We have our own incinerator. That's where all this stuff goes.
Connie McManus
Utah Veterinary Diagnostics Laboratory
Utah State University
Logan, UT
Phone: 435/797-1891
fax: 435/797-2805
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Ernestine Middleton
Sent: Monday, July 26, 2004 6:03 PM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] paraffin blocks
Hi;
I like to know how do other Histology Lab. get rid of paraffin blocks
that are 20+years. Do you have a medical waste company destroy them or
do you do it your self?
Thank you for answer me as quickly as possible.
Ernestine Middleton, Manager
Montefiore Med. Ct.
Bronx, NY
914-920-4157
---------------------------------
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------------------------------
Message: 22
Date: Tue, 27 Jul 2004 09:54:50 -0500
From: mari.ann.mailhiot <@t> leica-microsystems.com
Subject: Re: [Histonet] soften formalin fixed samples
To: Leclerc Jocelyne <leclercj <@t> vetanat.unizh.ch>
Cc: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OF9427D624.F3615132-ON86256EDE.0050F4C3-86256EDE.0051D9BE <@t> e-leica.com>
Content-Type: text/plain; charset=iso-8859-1
Jocelyne
You can soften your specimens a bit by soaking them on ice and water. You
will loose some of the specimen when you take your first sections. The ice
water will swell the tissue a bit. You could also soak the blocks on
glycerin, or liquid dish soap.
You may what to check your processing schedule of your specimens. If you
have too many 100% alcohols this will definitely cause hard tissue. You may
be taking bound water out of the tissue. You may also check to see if you
have heat on during some of your processing steps. To much heat may also
dry your samples depending on the size of the samples.
Hope this helps.
Mari Ann Mailhiot BA HT ASCP
Application Specialist
Leica Technical Assistance Center
800 248 0123 x7267
847 236 3063 fax
mari.ann.mailhiot <@t> leica-microsystems.com
www.leica-microsystems.com
Leclerc Jocelyne
<leclercj <@t> vetanat.unizh.ch> To:
<Histonet <@t> lists.utsouthwestern.edu>
Sent by: cc:
histonet-bounces <@t> lists.utsouth Subject:
[Histonet] soften formalin fixed samples
western.edu
07/27/2004 04:16 AM
Good morning everybody!
I have a problem with my formalin fixed samples: they are just too hard.
Now
I wonder if there is a possibility to soften or "remoisture" them.
I would be very pleased if anyone of you felt able to help me.
Thank you and best regards,
Jocelyne
--
VetSuisse Fakultät
Veterinär-Anatomisches Institut
Winterthurerstr. 260
8057 Zürich
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Message: 23
Date: Tue, 27 Jul 2004 09:13:27 -0600
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: Re: [Histonet] soften formalin fixed samples
To: Leclerc Jocelyne <leclercj <@t> vetanat.unizh.ch>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.0.20040727091008.01b12bf0 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="iso-8859-1"; format=flowed
First, check you tissue processing schedule, it may be too long -
overexposure to alcohols removes too much bound water (bound to proteins)
rather than just free water, over exposure to xylene also hardens tissue,
and heat of paraffin is very drying. If you samples are very tiny, you can
have shorter processing schedule to prevent drying out of tissue.
Second, you can face or trim the block, and place it face down on a block
of ice with water on top of ice, or just use ice water. This has been
discussed recently on Histonet (at great length) - go to archives and do a
search for more hints.
At 03:16 AM 7/27/2004, you wrote:
>Good morning everybody!
>
>I have a problem with my formalin fixed samples: they are just too hard.
Now
>I wonder if there is a possibility to soften or "remoisture" them.
>I would be very pleased if anyone of you felt able to help me.
>
>Thank you and best regards,
>
>Jocelyne
>--
>VetSuisse Fakultät
>Veterinär-Anatomisches Institut
>Winterthurerstr. 260
>8057 Zürich
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
------------------------------
Message: 24
Date: Tue, 27 Jul 2004 16:26:22 +0100
From: "Marshall Terry Dr, Consultant Histopathologist"
<Terry.Marshall <@t> rothgen.nhs.uk>
Subject: RE: [Histonet] soften formalin fixed samples
To: "Gayle Callis" <gcallis <@t> montana.edu>, "Leclerc Jocelyne"
<leclercj <@t> vetanat.unizh.ch>, <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<FE2DB935F8BBB546B8A1BBF3459C5A1F02DC9EBD <@t> LIL.xRothGen.nhs.uk>
Content-Type: text/plain; charset="iso-8859-1"
Gayle,
We are having trouble at the moment with poor clearing, giving a grey cast,
and sometimes a steely opaque look to things. Do you think that actually
reducing processing times in alcohol and xylene may help, or is this as
absurd as it sounds?
Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
Consultant Pathologist
Rotherham General Hospital
South Yorkshire
England
terry.marshall <@t> rothgen.nhs.uk
-----Original Message-----
From: Gayle Callis [mailto:gcallis <@t> montana.edu]
Sent: 27 July 2004 16:13
To: Leclerc Jocelyne; Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] soften formalin fixed samples
First, check you tissue processing schedule, it may be too long -
overexposure to alcohols removes too much bound water (bound to proteins)
rather than just free water, over exposure to xylene also hardens tissue,
and heat of paraffin is very drying. If you samples are very tiny, you can
have shorter processing schedule to prevent drying out of tissue.
Second, you can face or trim the block, and place it face down on a block
of ice with water on top of ice, or just use ice water. This has been
discussed recently on Histonet (at great length) - go to archives and do a
search for more hints.
At 03:16 AM 7/27/2004, you wrote:
>Good morning everybody!
>
>I have a problem with my formalin fixed samples: they are just too hard.
Now
>I wonder if there is a possibility to soften or "remoisture" them.
>I would be very pleased if anyone of you felt able to help me.
>
>Thank you and best regards,
>
>Jocelyne
>--
>VetSuisse Fakultät
>Veterinär-Anatomisches Institut
>Winterthurerstr. 260
>8057 Zürich
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 25
Date: Tue, 27 Jul 2004 10:56:18 -0500
From: "Winters, Bert" <BWinters <@t> NCH.ORG>
Subject: [Histonet] stain for mast cells
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <270614B321ACB44D8C1D91F4F921FDC3627776 <@t> NCH01EX02.nch.org>
Content-Type: text/plain; charset="iso-8859-1"
We have been using an alcian blue stain at pH 0.4 to demonstrate mast cells.
Our pathologist would like to use a giemsa stain for mast cells. If someone
knows a good stain for mast cells could you let me know. Either a giemsa
stain or any other type would be appreciated.
------------------------------
Message: 26
Date: Tue, 27 Jul 2004 12:43:12 -0400
From: Stanley.Lupo <@t> gsk.com
Subject: [Histonet] I have not received any histonet mail in a while
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OFE0C69848.C449C666-ON85256EDE.005BA3DA-85256EDE.005C4FD8 <@t> sb.com>
Content-Type: text/plain; charset=us-ascii
Stanley.Lupo <@t> GSK.Com
Safety Assessment
Mail Code: UE0461
Phone: 610 270-7340
Fax: 610 270-7202
Please resubscribe me to Histonet. I have stopped receiving Histonet
email.
Thanks.
Stan
------------------------------
Message: 27
Date: Tue, 27 Jul 2004 12:54:53 -0400
From: Dana Settembre <settembr <@t> umdnj.edu>
Subject: Re: [Histonet] AE 13 stain?
To: AFeatherstone <@t> KaleidaHealth.Org,
histonet <@t> lists.utsouthwestern.edu, emmie222 <@t> yahoo.com
Message-ID: <s10650c4.046 <@t> smtpnpc.umdnj.edu>
Content-Type: text/plain; charset=US-ASCII
Emily,
I first thought that you meant cytokeratin AE1 and AE3, a commonly used
cytokeratin antibody cocktial.
Just as Andi Kappeler mentioned. But now, Michael Fredrickson has some
specific info about pilar cytokeratin. ?
Dana
>>> "Featherstone, Annette" <AFeatherstone <@t> KaleidaHealth.Org> 7/27/2004
7:28:34 AM >>>
Does anyone know what AE-13 is? Is it an immunostain, and what is it
for?
Annette Featherstone HT/MLT
-----Original Message-----
From: Emily [mailto:emmie222 <@t> yahoo.com]
Sent: Monday, July 26, 2004 13:47
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Securline markers
We have been having a major problem the Securline markers for about a
year -
they are pretty much dry when we get them. The company that
manufactures
them told me to add xylene to each marker. It works most of the time,
but it
takes up a lot of time to add the xylene. My feeling is that I
shouldn't
have to do that anyway - they should just make a marker that actually
works.
I have tried out many other pens/markers and have found none to be
satisfactory. The ink either fades too much during processing or just
totally washes off. Does anyone have any similar issues? Does anyone
have
any ideas or suggestions?
_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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End of Histonet Digest, Vol 8, Issue 39
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