[Histonet] Conundrum

[Joe Nocito]

Christian,
We have had recent problems with tissue floating off the slides after
decloaking them and even after performing heat retrieval on the Ventana XT.
We have tried many different slides and still continue to do so. The best
that I've come across is from StatLab cat # 318. There's a catch to ordering
them though.  Their number is 800-442-3573. Tell them Joe sent you. I'm in
the middle of testing some slides from Erie Scientific, but haven't
completed the testing yet.

Joe Nocito, BS, HT(ASCP) QIHC
Histology Manager
Pathology Reference Lab
San Antonio, TX


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Christian
Franci
Sent: Friday, July 23, 2004 10:47 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Conundrum

I'm in somewhat of a pickle...
It seems that my PEFF sections keep floating away from the slides when I do
HIER using my usual pressure cooker method in which they're submerged in
buffer. Never had this problem before...

(I've always used a pressure cooker. Have never tried other methods.)
Can steaming them instead be a better way to avoid this?  If so, how do you
go about it?  any tricks of the trade?

My suspicion is that the formalin concentration that was used to fix the
tissues was off. (Could that be the case?)

Of course, these are precious study samples for a paper so... anyone have
any ideas how I can save them?  or am I out of luck?

Much gratitude for any insights that might help!
Cheers
Chris

=====
"Eliminate all other factors and, the one which remains MUST be the
truth".-Sherlock Holmes-




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