[Histonet] endothelial cell staining

[Elizabeth Chlipala]

Jerry

You can use PECAM-1 (M-20)  from Santa Cruz.  It is a goat polyclonal
and will work on formalin fixed paraffin embedded material.  I have used
it on mouse tissue with good results.  I have images and a protocol
available if you need one.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Histology Laboratory, LLC
P.O. Box 18592 
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
lizchlipala <@t> premierhistology.com
www.premierhistology.com
 
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jeremiah
Lyons
Sent: Friday, July 23, 2004 8:23 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] endothelial cell staining

Hi,

I'm a PhD student who is looking for advice on endothelial cell staining
in mouse tumour tissue for the purpose of microvessel density
staining.Most of the available antibodies don't work on mouse tissue. At
the moment I'm trying Carnoy's fixed tissue (fixed for four hours) with
rabbit anti-human Von Willebrand factor. Any suggestions would be
greatly appreciated.

Jerry
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