[Histonet] cryostats

[Galbraith, Joe]

Juan:

Sounds like an adjustment issue on the holder.  Not certain what model of blade holder you have but some older Leica models have two screws aligned horizontally one on each side of the locking plate.  This style was harder to adjust.  We replaced our blade holder on the 1850 with a newer model holder just to get rid of that hassle.  The newer models have one central large screw with a tiny adjustment screw immediately behind it.  The latter type can be adjusted quite easily to allow smooth blade changes and yet still get a good tight grip.  If you let me know what type of holder you have I can probably be more specific about adjusting it.  You may also have problems with moisture and ice crystal formation if you are cleaning (disinfecting) the cryostat blade holder while it is cold.  Send me some more info and maybe I can help you out.

Joe Galbraith

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Gayle
Callis
Sent: Tuesday, July 20, 2004 1:40 PM
To: GUTIERREZ, JUAN; Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] cryostats


WE have two 1850's side by side and I have NEVER had problems with the 
blade holder even after two years use with both of them.  You need to find 
out from the Leica rep what is going wrong with your holder, something is 
out of whack.

We cut hundreds of frozen sections a year - in fact, rarely do paraffin for 
any immunohistochemistry.

At 09:31 AM 7/19/2004, you wrote:
>I have never used a H/B, but I would give it a try.  Try both before you 
>buy.  I have a Leica 1850 and it had to be replaced within two years.  The 
>current one is doing fine.  If you get a good one the Leica's are okay 
>except for the blade holder.  It has to be taken apart and adjusted every 
>time you change the blade( the pressure clip is to soft).  Now if you 
>could choose another company I would try Microm(Richard Allan).
>
>Juan C. Gutierrez, HT(ASCP)
>Histology Laboratory Supervisor
>Christus Santa Rosa Hospital
>333 N. Santa Rosa Ave.
>San Antonio, TX  78207
>(210)704-2533
>
>
>-----Original Message-----
>From: Kathleen Spencer [mailto:kspencer <@t> utmem.edu]
>Sent: Monday, July 19, 2004 9:51 AM
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] cryostats
>
>
>Hi all,
>
>Question: If you had to buy a new cryostat, and you had to chose between
>a Hacker Bright or a Leica, which would you purchase, and please tell me
>why. Mainly I would love to hear from anyone that has had these in the
>last 10 years.
>I really need your input on this, detailed input, especially in regards
>to when you bought the machine that you do not like and how long before
>it started giving you trouble.
>
>Thanks in advance,
>
>Kathleen Spencer HT (ASCP)
>Lab Manager/ LCM Supervisor
>UTHSC
>
>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)



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