[Histonet] GFAP Protocol

Jan Shivers shive003 <@t> umn.edu
Tue Jul 20 08:03:19 CDT 2004


Sure.  I use Dako's GFAP on their autostainer all the time in veterinary
diagnostics.  It requires NO tissue pretreatment.  The antibody can be used
at a very high dilution (right now I'm using 1:1200 dilution of anti-GFAP
with a DAKO EnVision+/Rb detection system).

Jan Shivers
University of Minnesota Veterinary Diagnostic Lab
St. Paul, MN, USA
612-624-7297
shive003 <@t> tc.umn.edu

----- Original Message ----- 
From: "Onnasch, Hanne" <Hanne.Onnasch <@t> agriculture.gov.ie>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, July 20, 2004 3:59 AM
Subject: [Histonet] GFAP Protocol



Hi All,

I'm looking for a protocol for IHC staining for GFAP on paraffin sections.
Does anyone have experience with using the Dako detection kit and an
automated system?

Regards,
Hanne


Hanne Onnasch
Research Officer
Central Veterinary Research Laboratory
Abbotstown
Castleknock
Dublin 15

phone: +353(0)16072583
Fax: +353(0)16072604
hanne.onnasch <@t> agriculture.gov.ie




**********************************************************************
***********  Department of Agriculture and Food ***************

The information contained in this email and in any
attachments is confidential and is designated solely
for the attention and use of the intended recipient(s).
This information may be subject to legal and professional
privilege.  If you are not an intended recipient of
this email, you must not use, disclose, copy,
distribute or retain this message or any part of it.
If you have received this email in error, please
notify the sender immediately and delete all copies of
this email from your computer system(s).
**********************************************************************


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet





More information about the Histonet mailing list