[Histonet] lymph node clearing, hardening

[Featherstone, Annette]

Anyone using a homemade lymph node clearing and hardening solution that does
not contain chloroform or ether?
Annette Featherstone

-----Original Message-----
From: Stephen.Eyres <@t> sanofi-synthelabo.com
[mailto:Stephen.Eyres <@t> sanofi-synthelabo.com]
Sent: Monday, July 19, 2004 09:05
To: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Bouin's alternative






Hi Marlene,

We use Davidson's fluid, which is a mixture of Alcohol, formalin and acetic
acid.

Steve


 

                      Marlene Nardi

                      <mcc12 <@t> cornell.edu>                    To:
histonet <@t> lists.utsouthwestern.edu

                      Sent by:                               cc:

                      histonet-bounces <@t> lists.utsouth         Subject:
[Histonet] Bouin's alternative

                      western.edu

 

 

                      19/07/2004 13:17

 

 





We currently use Bouin's solution to fix animal eyes (good retinal
preservation), to fix animal reproductive tissues (prevents shrinkage of
testes), and as the post-fix in the Masson Trichrome stain.  Any ideas on a
less hazardous alternative solution for any of these uses?  For the
Trichrome stain I have tried the commercial Bouin's substitute, Gram's
iodine and citrate buffer with limited/no success.
thank you in advance,
Marlene


=====
Marlene C. Nardi
Technical Services Supervisor
Histology Laboratory
New York State College of Veterinary Medicine
Cornell University
Department of Biomedical Sciences
S2-124 Schurman Hall
Ithaca, NY 14853
mailto:mcc12 <@t> cornell.edu
ph. 607-253-3314 or 607-253-3303
FAX  607-253-3357



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