[Histonet] Dezenkerize

Karen Wittler kwittle <@t> jhmi.edu
Mon Jul 19 14:10:05 CDT 2004


Sorry It took me so long to respond. We dissolve iodine in xylene. We
have few B5 specimens so we use a coplin jar and treat a couple of
slides at a time for five minutes and then hang them on our automated
stainer at the first deparaffinizing xylene. As far as the amount of
iodine in xylene, It's like a palm full of parsley, season to taste,
whatever works.

Karen Wittler, HT(ASCP)
Johns Hopkins Hospital
Baltimore, MD




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