[Histonet] Bouin's alternative

[Stephen.Eyres <@t> sanofi-synthelabo.com]

Hi Marlene,

We use Davidson's fluid, which is a mixture of Alcohol, formalin and acetic
acid.

Steve


                                                                                                                                                     
                      Marlene Nardi                                                                                                                  
                      <mcc12 <@t> cornell.edu>                    To:      histonet <@t> lists.utsouthwestern.edu                                              
                      Sent by:                               cc:                                                                                     
                      histonet-bounces <@t> lists.utsouth         Subject: [Histonet] Bouin's alternative                                                 
                      western.edu                                                                                                                    
                                                                                                                                                     
                                                                                                                                                     
                      19/07/2004 13:17                                                                                                               
                                                                                                                                                     
                                                                                                                                                     




We currently use Bouin's solution to fix animal eyes (good retinal
preservation), to fix animal reproductive tissues (prevents shrinkage of
testes), and as the post-fix in the Masson Trichrome stain.  Any ideas on a
less hazardous alternative solution for any of these uses?  For the
Trichrome stain I have tried the commercial Bouin's substitute, Gram's
iodine and citrate buffer with limited/no success.
thank you in advance,
Marlene


=====
Marlene C. Nardi
Technical Services Supervisor
Histology Laboratory
New York State College of Veterinary Medicine
Cornell University
Department of Biomedical Sciences
S2-124 Schurman Hall
Ithaca, NY 14853
mailto:mcc12 <@t> cornell.edu
ph. 607-253-3314 or 607-253-3303
FAX  607-253-3357



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