[Histonet] Fixative and H2O2 for frozen

[SMITH,REBEKAH FELICIA]

I second that. My prof always told me its important to make up the 
peroxide new every day.
                                     Rebekah Smith
On Sat Jul 17 17:52:39 EDT 2004, Patsy Ruegg <pruegg <@t> ihctech.net> 
wrote:

> I do use freshly prepared fixative and h202 each time.
> Patsy
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of 
> Jorge
> Ivan Zapata Valencia
> Sent: Saturday, May 15, 2004 12:46 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Fixative and H2O2 for frozen
> 
> 
> Hello everybody
> a technical question: may I use the same acetone:etanol mix to 
> fix samples
> different days, or do I have to prepare it feshly every day?? The 
> same with
> the H2O2 PBS solution for endogenous peroxidase blocking. Is it 
> mandatory to
> use fresh one?
> Thank You very much
> 
> jorge ivan zapata
> 
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--
SMITH,REBEKAH FELICIA
"You are a child of the universe, no less than the trees and the 
stars
You have a right to be here and whether or not it is clear to you, 
no doubt the universe is unfolding as it should. Therefore be at 
peace with G-d, whatever you conceive Him to be. And whatever your 
labors and aspirations,in the noisy confusion of life, keep peace 
in your soul.-Max Ehrmann,"Desiderata"