[Histonet] protocol for freezing fixed rat skeletal muscle

[zhangl]

Hi Histontters,

Thanks for the response to my question "mapping capillary perfusion pattern".
  Here I have another question need your help. I am going to perfuse rat 
hindlimb with BSA+FITC-dextran, then to locate the FITC-dextran in frozen 
muscle tissue. To prevent FITC-dextran from diffusing out of tissue when I 
mount the tissue onto a slide, will some fixation before freezing the 
tissue be helpful? Can anyone kindly share a good protocol for freezing 
fixed muscle tissue? fixative? how long the fixation? any cryoprotection? 
By the way, I wonder if anyone knows whether the FITC will bleach during 
the fixation, freezing process?  Thanks in advance.

Lei Zhang
Biochemistry, medicine school
University of Tasmania, Australia
61 03 6226 2669
zhangl <@t> utas.edu.au