[BULK] - [Histonet] thick and thin sections

[Pamela Marcum]

One other issue is overfilling the cassettes during embedding and having an
excess of rounded paraffin sticking up above the rim.  As this is more
rounded it will allow the block to slip and not fit well in the chuck.  Look
at the back and be sure the paraffin is level across the cassette back as
well as full only to the rim.

Thanks,

Pam Marcum 


> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Fred
> Underwood
> Sent: Friday, July 16, 2004 9:55 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [BULK] - [Histonet] thick and thin sections
>
>
> Maybe her clamp is gooped up with paraffin waste and the clamp does not
> spring shut tightly.  It may also be the brand of cassettes she is
> using.  Some of them have a little more give, which can be minimized by
> making sure the cassette is filled to the brim with paraffin at
> embedding.
>
> Fred
>
> >>> <Pathologyarts <@t> aol.com> 07/15/04 05:43PM >>>
> i have a friend who says she is getting thick and thin sections because
> the
> block can slide laterally in the block holder. using a 2025 my blocks
> also move
> but i don't have any trouble with the thick and thin sections. the side
> to
> side movement doesn't seem to make sense to me, anyone have any input,
> ever seen
> anything like this?
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