[Histonet] kupffer cell

[Jose Luis Palazon Fernandez]

Dear Histonetters

First I would like to say hello to all of you after a time out of the histonett. 
I would like to stablish the presence of kupffer cells in a fish species. I know that one of the procedures consist of inyecting ink to the animal and then the cells appear black in the slides but I dont know the detailed procedure used. can any of you please let me know the detailed procedure indicating if some changes in tissue embedding is necesary?. It does not matter if the procedure if for a mammalian I will adapt it to fish.

thanks in advance

José Luis