[Histonet] Eosin Staining
Jaimie Hoh
jaimie_hk <@t> yahoo.co.uk
Thu Jul 15 02:29:44 CDT 2004
Hi Jennifer,
This is what i normally do when I overstained with
Eosin:
After rehydrating the specimen with Xylene and then
descending concentrations of ethanol;
1. if the eosin is aqueous base, then i'll let the
slides for a few minutes in water until the intensity
of the Eosin is correct.
2. If the Eosin is Alcoholic, then Ill let the slides
in 95% Ethanol for a few minutes until the Eosin fade
to the intensity desired.
Jaimie
Research Officer
Singapore Eye Research Institute
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