[Histonet] Eosin Staining

Jaimie Hoh jaimie_hk <@t> yahoo.co.uk
Thu Jul 15 02:29:44 CDT 2004

Hi Jennifer,

This is what i normally do when I overstained with

After rehydrating the specimen with Xylene and then
descending concentrations of ethanol;
 1. if the eosin is aqueous base, then i'll let the
slides for a few minutes in water until the intensity
of the Eosin is correct. 
2. If the Eosin is Alcoholic, then I’ll let the slides
in 95% Ethanol for a few minutes until the Eosin fade
to the intensity desired.

Research Officer
Singapore Eye Research Institute

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