[Histonet] Storage of H&E slides

[Barry R Rittman]

Margaret
I do not know of any reference related specifically to this. It would be
difficult because, as has been pointed out, fading may depend on the
type of hematoxylin, type of eosin procedures, clearing agents,
mountants and storage conditions and even small differences may alter
the end product.
The fact that there are slides from the late 1800s that are still
stained with dyes such as carmine and some with hematoxylin attests to
the fact that hematoxylin at least can survive well. Eosin seems a bit
more variable. There is considerable variation between slides that have
been treated identically. I have slide that I prepared 40 years ago that
are still well stained with H and E and others prepared at the same time
using the same processing and staining that have faded.
I believe that the greatest concern with storage should be the adhesion
of cover glasses. Once air creeps under the cover glass the sections
fade.
Hope this helps.
Barry

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of RCPA QAP
Pty Ltd
Sent: Tuesday, July 13, 2004 5:13 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Storage of H&E slides

Dear all
I am looking for a reference to support the statement that H&E slides
are 
stable (ie can be stored without losing the quality of the staining).
Does 
anyone know of one?
Margaret

RCPA Quality Assurance Programs Pty Ltd
Anatomical Pathology
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Burwood Vic 3125
Australia
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